Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1996-8-6
pubmed:abstractText
Metanephric mesenchyme gives rise to both the epithelial cells of the nephron and the stromal cells of the mature kidney. The function of the stroma. in kidney morphogenesis is poorly understood. We have generated mice with a null mutation in the Winged Helix (WH) transcription factor BF-2 to examine its function during development. BF-2 expression within the developing kidney is restricted to the stromal cell lineage. Homozygotes die within the first 24 hr after birth with abnormal kidneys. Mutant kidneys are small, fused longitudinally, and rotated 90 degrees ventrally. Histological examination reveals a smaller collecting system, numerous large condensations of mesenchyme, and a decrease in the number of nephrons. Using molecular markers we show that induction and condensation of the nephrogenic mesenchyme occurs normally in mutant. The disruption of BF-2 reduces the rate of differentiation of the condensed mesenchyme into tubular epithelium, as well as the rate of growth and branching of the ureter and collecting system. Our findings demonstrate that BF-2 and stromal cells have essential functions during kidney morphogenesis. Furthermore, they suggest that BF-2 controls the production, by the stroma, of signals or factors that are required for the normal transition of induced mesenchyme into tubular epithelium and full growth and branching of the collecting system.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0890-9369
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1467-78
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8666231-Animals, pubmed-meshheading:8666231-Base Sequence, pubmed-meshheading:8666231-DNA-Binding Proteins, pubmed-meshheading:8666231-Embryonic Induction, pubmed-meshheading:8666231-Epithelium, pubmed-meshheading:8666231-Forkhead Transcription Factors, pubmed-meshheading:8666231-Gene Expression Regulation, Developmental, pubmed-meshheading:8666231-Homozygote, pubmed-meshheading:8666231-Kidney, pubmed-meshheading:8666231-Kidney Tubules, Collecting, pubmed-meshheading:8666231-Mesoderm, pubmed-meshheading:8666231-Mice, pubmed-meshheading:8666231-Mice, Transgenic, pubmed-meshheading:8666231-Models, Biological, pubmed-meshheading:8666231-Molecular Sequence Data, pubmed-meshheading:8666231-Morphogenesis, pubmed-meshheading:8666231-Mutation, pubmed-meshheading:8666231-Nephrons, pubmed-meshheading:8666231-Nerve Tissue Proteins, pubmed-meshheading:8666231-Stromal Cells, pubmed-meshheading:8666231-Ureter
pubmed:year
1996
pubmed:articleTitle
Essential role of stromal mesenchyme in kidney morphogenesis revealed by targeted disruption of Winged Helix transcription factor BF-2.
pubmed:affiliation
Cell Biology Program and Division of Endocrinology, Cornell University Graduate School of Medical Sciences, Memorial Sloan-Kettering Cancer Center, New York, New York 10021, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't