Linked Life Data

8665080

Source:http://linkedlifedata.com/resource/pubmed/id/8665080

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-8-8
pubmed:abstractText
The ability to replace damaged myocardial tissue with new striated muscle would constitute a major advance in the treatment of diseases that irreversibly injure cardiac muscle cells. The creation of focal grafts of skeletal muscle has been reported following the intramural injection of skeletal myoblasts into both normal and injured myocardium. The goals of this study were to determine whether skeletal myoblast-derived cells can be engrafted into the murine heart following arterial delivery. The murine heart was seeded with genetically labeled C2C12 myoblasts introduced into the arterial circulation of the heart via a transventricular injection. A transventricular injection provided access to the coronary and systemic circulations. Implanted cells were characterized using histochemical staining for beta-galactosidase, immunofluorescent staining for muscle-specific antigens, and electron microscopy. Initially the injected cells were observed entrapped in myocardial capillaries. One week after injection myoblasts were present in the myocardial interstitium and were largely absent from the myocardial capillary bed. Implanted cells underwent myogenic development, characterized by the expression of a fast-twitch skeletal muscle sarcoendoplasmic reticulum calcium ATPase (SERCA1) and formation of myofilaments. Four months following injection myoblast-derived cells began to express a slow-twitch/cardiac protein, phospholamban, that is normally not expressed by C2C12 cells in vitro. Most surprisingly, regions of close apposition between LacZ labeled cells and native cardiomyocytes contained structures that resembled desmosomes, fascia adherens junctions, and gap junctions. The cardiac gap junction protein, connexin43, was localized to some of the interfaces between implanted cells and cardiomyocytes. Collectively, these findings suggest that arterially delivered myoblasts can be engrafted into the heart, and that prolonged residence in the myocardium may alter the phenotype of these skeletal muscle-derived cells. Further studies are necessary to determine whether arterial delivery of skeletal myoblasts can be developed as treatment for myocardial dysfunction.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0963-6897
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
77-91
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:8665080-Animals, pubmed-meshheading:8665080-Biological Markers, pubmed-meshheading:8665080-Calcium-Binding Proteins, pubmed-meshheading:8665080-Cell Line, pubmed-meshheading:8665080-Connexin 43, pubmed-meshheading:8665080-Escherichia coli, pubmed-meshheading:8665080-Graft Survival, pubmed-meshheading:8665080-Heart, pubmed-meshheading:8665080-Male, pubmed-meshheading:8665080-Mice, pubmed-meshheading:8665080-Mice, Inbred C3H, pubmed-meshheading:8665080-Microscopy, Electron, pubmed-meshheading:8665080-Muscle, Skeletal, pubmed-meshheading:8665080-Muscle Fibers, Skeletal, pubmed-meshheading:8665080-Recombinant Proteins, pubmed-meshheading:8665080-Time Factors, pubmed-meshheading:8665080-Transfection, pubmed-meshheading:8665080-Transplantation, Heterotopic, pubmed-meshheading:8665080-beta-Galactosidase
pubmed:articleTitle
Arterial delivery of genetically labelled skeletal myoblasts to the murine heart: long-term survival and phenotypic modification of implanted myoblasts.
pubmed:affiliation
Peter Belfer Cardiac Laboratory, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't