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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1996-8-7
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pubmed:abstractText |
The molecular basis of the regulation of cardiac L-type calcium channel activity by cAMP-dependent protein kinase (cA-PK) remains unclear. Direct cA-PK-dependent phosphorylation of the bovine ventricular alpha1 subunit in vitro has been demonstrated in microsomal membranes, detergent extracts and partially purified (+)-[3H]PN 200-110 receptor preparations. Two 32P-labeled phosphopeptides, derived from cyanogen bromide cleavage, of 4.7 and 9.5 kDa were immunoprecipitated specifically by site-directed antibodies against the rabbit cardiac alpha1 subunit amino acid sequences 1602-1616 and 1681-1694, respectively, consistent with phosphorylation at the cA-PK consensus sites at Ser(1627) and Ser(1700). No phosphopeptide products consistent with phosphorylation at three other C-terminal cA-PK consensus phosphorylation sites (Ser(1575), Ser(1848) and Ser(1928)) were identified using similar procedures suggesting that these sites are poor substrates for this kinase. Ser(1627) and Ser(1700) may represent sites of cA-PK phosphorylation involved in the physiological regulation of cardiac L-type calcium channel function.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Cyanogen Bromide,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP-Dependent Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Isradipine,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoserine
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0006-3002
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
11
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pubmed:volume |
1281
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
205-12
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8664319-Amino Acid Sequence,
pubmed-meshheading:8664319-Animals,
pubmed-meshheading:8664319-Binding Sites,
pubmed-meshheading:8664319-Calcium Channels,
pubmed-meshheading:8664319-Cattle,
pubmed-meshheading:8664319-Cyanogen Bromide,
pubmed-meshheading:8664319-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:8664319-Heart Ventricles,
pubmed-meshheading:8664319-Immunosorbent Techniques,
pubmed-meshheading:8664319-Isradipine,
pubmed-meshheading:8664319-Microsomes,
pubmed-meshheading:8664319-Molecular Sequence Data,
pubmed-meshheading:8664319-Peptide Fragments,
pubmed-meshheading:8664319-Phosphorylation,
pubmed-meshheading:8664319-Phosphoserine,
pubmed-meshheading:8664319-Rabbits
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pubmed:year |
1996
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pubmed:articleTitle |
Cyclic AMP-dependent protein kinase phosphorylates residues in the C-terminal domain of the cardiac L-type calcium channel alpha1 subunit.
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pubmed:affiliation |
Department of Medicine and Therapeutics, University of Leicester, Clinical Sciences Building, Leicester Royal Infirmary, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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