Linked Life Data

8664308

Source:http://linkedlifedata.com/resource/pubmed/id/8664308

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1996-8-2
pubmed:abstractText
Using the technique of laser flash photolysis of caged ATP, we have suggested that, under nearly isometric conditions, the unitary distance of myofilament sliding per ATP molecule (myosin head powerstroke) is about 10 nm. To give further information about the mechanism of myofilament sliding, we studied the effect of inorganic phosphate (Pi) and ADP on the photoreleased ATP-induced shortening of single glycerinated muscle fibers under very small external loads. Both the velocity and the distance of the myofilament sliding induced by 150 microM ATP increased by Pi (20 mM), and decreased by ADP (0.4 mM). On the other hand, Pi and ADP showed no significant effect on the myofilament sliding induced by 100 and 75 microM ATP. The potentiating effect of Pi on the myofilament sliding with 150 microM ATP can be explained as being due to the increase in population of AM.ADP.Pi with corresponding decrease in population of AM.ADP, and also the increase in population of M.ADP.Pi and M.ATP. Meanwhile, the inhibitory effect of ADP can be simply accounted for to be due to an accumulation of AM.ADP that already finished their force generating process. The ineffectiveness of Pi and ADP on the myofilament sliding with 100 and 75 microM ATP is consistent with the view that it is caused by almost synchronized single myosin head powerstrokes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
13
pubmed:volume
1274
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
89-93
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Effect of inorganic phosphate and ADP on the myofilament sliding induced by laser flash photolysis of caged ATP.
pubmed:affiliation
Department of Physiology, School of Medicine, Teikyo University, Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't