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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
29
|
| pubmed:dateCreated |
1996-9-12
|
| pubmed:databankReference | |
| pubmed:abstractText |
We have isolated and begun characterization of the quail slow myosin heavy chain (MyHC) 3 gene, the first reported avian slow MyHC gene. Expression of slow MyHC 3 in skeletal muscle is restricted to the embryonic period of development, when the fiber pattern of future fast and slow muscle is established. In embryonic hindlimb development, slow MyHC 3 gene expression coincides with slow muscle fiber formation as distinguished by slow MyHC-specific antibody staining. In addition to expression in embryonic appendicular muscle, slow MyHC 3 is expressed continuously in the atria. Transfection of slow MyHC 3 promoter-reporter constructs into embryonic myoblasts that form slow MyHC-expressing fibers identified two regions regulating expression of this gene in skeletal muscle. The proximal promoter, containing potential muscle-specific regulatory motifs, permits expression of a reporter gene in embryonic slow muscle fibers, while a distal element, located greater than 2600 base pairs upstream, further enhances expression 3-fold. The slow muscle fiber-restricted expression of slow MyHC 3 during embryonic development, and expression of slow MyHC 3 promoter-reporter constructs in embryonic muscle fibers in vitro, makes this gene a useful marker to study the mechanism establishing the slow fiber lineage in the embryo.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
19
|
| pubmed:volume |
271
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
17047-56
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8663323-Amino Acid Sequence,
pubmed-meshheading:8663323-Animals,
pubmed-meshheading:8663323-Base Sequence,
pubmed-meshheading:8663323-Chick Embryo,
pubmed-meshheading:8663323-Chickens,
pubmed-meshheading:8663323-Cloning, Molecular,
pubmed-meshheading:8663323-Embryo, Nonmammalian,
pubmed-meshheading:8663323-Exons,
pubmed-meshheading:8663323-Gene Expression Regulation, Developmental,
pubmed-meshheading:8663323-Genomic Library,
pubmed-meshheading:8663323-Heart,
pubmed-meshheading:8663323-In Situ Hybridization,
pubmed-meshheading:8663323-Molecular Sequence Data,
pubmed-meshheading:8663323-Muscle, Skeletal,
pubmed-meshheading:8663323-Muscle Fibers, Fast-Twitch,
pubmed-meshheading:8663323-Muscle Fibers, Slow-Twitch,
pubmed-meshheading:8663323-Myocardium,
pubmed-meshheading:8663323-Myosin Heavy Chains,
pubmed-meshheading:8663323-Oligodeoxyribonucleotides,
pubmed-meshheading:8663323-Promoter Regions, Genetic,
pubmed-meshheading:8663323-Quail,
pubmed-meshheading:8663323-RNA, Messenger,
pubmed-meshheading:8663323-Rats,
pubmed-meshheading:8663323-Sequence Homology, Amino Acid,
pubmed-meshheading:8663323-Transcription, Genetic
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Isolation and characterization of an avian slow myosin heavy chain gene expressed during embryonic skeletal muscle fiber formation.
|
| pubmed:affiliation |
Department of Medicine, Stanford University School of Medicine, Stanford, California 94305-5306, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|