Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
24
|
| pubmed:dateCreated |
1996-8-20
|
| pubmed:abstractText |
GADD153, a ubiquitously expressed member of the CCAAT/enhancer-binding protein (C/EBP) family is induced by a wide variety of growth-arresting and DNA-damaging agents. Functionally, GADD153 has been postulated to act as a dominant-negative regulator of C/EBPs. Therefore we sought to gain evidence for interactions between GADD153 and other C/EBPs during cellular responses to stress. In this report we have demonstrated that treatment of rat pheochromocytoma PC12 cells with sodium arsenite leads to enhanced expression of C/EBP-beta and GADD153 (growth arrest and DNA damage inducible gene 153) but not other C/EBPs. Coimmunoprecipitation experiments provided evidence for the formation of endogenous GADD153-C/EBP-beta complexes in arsenite-treated cells. Additional experiments were performed to determine the role of such complexes in regulating GADD153 expression. Previous studies in our laboratory demonstrated that the GADD153 promoter contains a C/EBP binding site through which other C/EBPs interact to transactivate GADD153 expression in liver hepatoma cells. Here, we demonstrate that extracts prepared from arsenite-treated PC12 cells likewise show increased amounts of factors capable of binding to the GADD153-C/EBP site and that these complexes are comprised at least in part of C/EBP-beta. Forced expression of C/EBP-beta was found to be capable of transactivating the GADD153 promoter in PC12 cells cotransfected with plasmids expressing a GADD153 reporter gene and C/EBP-beta protein. However, overexpression of GADD153 inhibited the transactivation of the GADD153 promoter by C/EBP-beta. These findings provide evidence for an autoregulatory loop in which stress-induced GADD153 feeds back to attenuate GADD153 expression during the cellular response to stress.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arsenites,
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ddit3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Sp1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor CHOP,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/arsenite
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
14
|
| pubmed:volume |
271
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
14285-9
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8662954-Adrenal Gland Neoplasms,
pubmed-meshheading:8662954-Animals,
pubmed-meshheading:8662954-Arsenites,
pubmed-meshheading:8662954-Base Sequence,
pubmed-meshheading:8662954-Binding Sites,
pubmed-meshheading:8662954-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:8662954-Consensus Sequence,
pubmed-meshheading:8662954-Cricetinae,
pubmed-meshheading:8662954-DNA Damage,
pubmed-meshheading:8662954-DNA-Binding Proteins,
pubmed-meshheading:8662954-Molecular Sequence Data,
pubmed-meshheading:8662954-NF-kappa B,
pubmed-meshheading:8662954-Oligodeoxyribonucleotides,
pubmed-meshheading:8662954-PC12 Cells,
pubmed-meshheading:8662954-Pheochromocytoma,
pubmed-meshheading:8662954-Promoter Regions, Genetic,
pubmed-meshheading:8662954-Rats,
pubmed-meshheading:8662954-Sp1 Transcription Factor,
pubmed-meshheading:8662954-Stress, Physiological,
pubmed-meshheading:8662954-Transcription, Genetic,
pubmed-meshheading:8662954-Transcription Factor CHOP,
pubmed-meshheading:8662954-Transcription Factors
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Physical and functional association between GADD153 and CCAAT/enhancer-binding protein beta during cellular stress.
|
| pubmed:affiliation |
Gene Expression and Aging Section, NIA, National Institutes of Health, Baltimore Maryland 21224, USA.
|
| pubmed:publicationType |
Journal Article
|