Linked Life Data

8662280

Source:http://linkedlifedata.com/resource/pubmed/id/8662280

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-4-8
pubmed:abstractText
45Ca2+ flux and single channel measurements have related that the Ca2+ release channel/ryanodine receptor complex of striated muscle is regulated by micromolar cytoplasmic Ca2+. The effect of luminal Ca2+, however, remains controversial. In the experiments presented here, we reconstituted the isolated Ca2+ release channel of rabbit skeletal muscle sarcoplasmic reticulum into planar lipid bilayers in the presence of symmetrical K+ solutions. Using K+ as the charge carrier, we were able to examine the effect of changes in luminal calcium in the micro- to millimolar range. In the presence of activating cytoplasmic Ca2+, the release channel was activated and inactivated in a concentration-dependent manner by luminal Ca2+. Since increasing cytoplasmic EGTA concentrations shifted the dependence of channel open probability on luminal Ca2+ to higher Ca2+ concentrations, it is suggested that luminal Ca2+ exerts ist regulating effect by acting on Ca2+ binding sites accessible from the cytoplasmic side of the channel.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0031-6768
pubmed:author
pubmed:issnType
Print
pubmed:volume
432
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
155-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Regulation of the purified Ca2+ release channel/ryanodine receptor complex of skeletal muscle sarcoplasmic reticulum by luminal calcium.
pubmed:affiliation
Department of Applied Physiology, University of Ulm, D-89069 Ulm, Germany.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't