Linked Life Data

8661292

Source:http://linkedlifedata.com/resource/pubmed/id/8661292

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1996-8-16
pubmed:abstractText
The hydroxyl and some alkoxyl spin adducts of 5,5-dimethyl-1-pyrroline 1-oxide (DMPO) are difficult to assign due to the remarkable similarity of their EPR spectra. The utility of resolving superhyperfine (SHF) structure followed by computer simulations has been demonstrated to assist in the assignment of EPR spectra with close values of hyperfine splitting constants, e.g., DMPO/ .OH and DMPO/.OR. Here, .OR is the alkoxyl radical derived from thermal decomposition of 2,2' -azobis (2-amidinopropane) hydrochloride (AAPH). In addition, two other spin traps, derivatives of 2H-imidazole 1-oxide, namely, 2,2,4-trimethyl-2H-imidazole 1-oxide (TMIO) and 2,2-dimethyl-4-phenyl-2H-imidazole 1-oxide (DMPIO), have been used in a model study to develop a procedure for distinguishing between oxygen-centered spin adducts. These results are compared with those for DMPO. TMIO and DMPIO spin traps provide more distinguishable individual spectra with .OH and AAPH-derived .OR radicals than the DMPO spin trap. The formation of DMPO/.OR(AAPH) and DMPIO/ .OR(AAPH) spin adducts was confirmed by mass spectrometry. The comparison of spin trapping by DMPO and 2H-imidazole 1-oxides using typical biological sources of other oxygen-centered radicals reveals application limits of these spin traps. For example, 2H-imidazole 1-oxides do not form superoxide spin adducts in the xanthine/xanthine oxidase system. Also, for the first time, experimental evidence is presented for SHF structure in spectra of TMIO and DMPIO spin adducts with .OH/.OD and .CH3/ .CD3 radical species.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1064-1866
pubmed:author
pubmed:issnType
Print
pubmed:volume
111
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
272-80
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Oxygen-centered spin adducts of 5,5-dimethyl-1-pyrroline N-oxide (DMPO) and 2H-imidazole 1-oxides.
pubmed:affiliation
Department of Biochemistry, University of Kansas, Lawrence 66045-2106, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't