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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1996-8-1
|
| pubmed:abstractText |
The central variable region (CVR) of the African swine fever virus (ASFV) genome is contained within the 9-RL open reading frame (ORF). ORF 9-RL of the ASFV isolate Malawi Lil-20/1 predicts a protein of 614 amino acids with amino- and carboxy-terminal hydrophobic regions and a centrally located hydrophilic region. The CVR of the genome, located centrally within this ORF, is 372 bp and contains a 132-bp direct repeat. The translated CVR within ORF 9-RL contains 31 tandem tetramers, predominantly NADT, NANT, NVDT, and, in a few cases, CAST, GAST, or CADT. In vitro translation of 9-RL yielded a 94-kDa protein that was strongly reactive with convalescent pig serum while monospecific 9-RL antiserum identified a late viral protein of 94 kDa in ASFV-infected macrophages. The protein, detected by immunofluorescence staining with 9-RL antiserum, was distributed homogeneously throughout the cytoplasm of infected Vero cells. 9-RL protein size varied among different viral isolates and among cell-culture-adapted viruses. Protein size increased proportionately with the degree of cell culture adaptation and was directly correlated with the size of the CVR present within the ORF (300-500 bp). Analysis of the number and composition of tandem tetramers present within the CVR of a given ASFV isolate may prove useful for identifying and/or grouping ASFV isolates.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0042-6822
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
220
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
20-7
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:8659112-African Swine Fever Virus,
pubmed-meshheading:8659112-Amino Acid Sequence,
pubmed-meshheading:8659112-Animals,
pubmed-meshheading:8659112-Base Sequence,
pubmed-meshheading:8659112-Cell Line,
pubmed-meshheading:8659112-Cercopithecus aethiops,
pubmed-meshheading:8659112-Cloning, Molecular,
pubmed-meshheading:8659112-Codon,
pubmed-meshheading:8659112-DNA Primers,
pubmed-meshheading:8659112-Gene Expression,
pubmed-meshheading:8659112-Genes, Viral,
pubmed-meshheading:8659112-Genetic Variation,
pubmed-meshheading:8659112-Macrophages,
pubmed-meshheading:8659112-Molecular Sequence Data,
pubmed-meshheading:8659112-Open Reading Frames,
pubmed-meshheading:8659112-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:8659112-Swine,
pubmed-meshheading:8659112-Vero Cells,
pubmed-meshheading:8659112-Viral Proteins,
pubmed-meshheading:8659112-Virus Replication
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Amino acid tandem repeats within a late viral gene define the central variable region of African swine fever virus.
|
| pubmed:affiliation |
Plum Island Animal Disease Center, ARS, USDA, Greenport, New York 11944-0848, USA.
|
| pubmed:publicationType |
Journal Article
|