Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1996-7-26
pubmed:databankReference
pubmed:abstractText
The drrC gene, cloned from the daunorubicin (DNR)- and doxorubicin-producing strain of Streptomyces peucetius ATCC 29050, encodes a 764-amino-acid protein with a strong sequence similarity to the Escherichia coli and Micrococcus luteus UvrA proteins involved in excision repair of DNA. Expression of drrC was correlated with the timing of DNR production in the growth medium tested and was not dependent on the presence of DNR. Since introduction of drrC into Streptomyces lividans imparted a DNR resistance phenotype, this gene is believed to be a DNR resistance gene. The drrC gene could be disrupted in the non-DNR-producing S. peucetius dnrJ mutant but not in the wild-type strain, and the resulting dnrJ drrC double mutant was significantly more sensitive to DNR in efficiency-of-plating experiments. Expression of drrC in an E. coli uvrA strain conferred significant DNR resistance to this highly DNR-sensitive mutant. However, the DrrC protein did not complement the uvrA mutation to protect the mutant from the lethal effects of UV or mitomycin even though it enhanced the UV resistance of a uvrA+ strain. We speculate that the DrrC protein mediates a novel type of DNR resistance, possibly different from the mechanism of DNR resistance governed by the S. peucetius drrAB genes, which are believed to encode a DNR antiporter.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-1369467, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-14090569, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-1537806, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-1592819, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-1612454, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-1655560, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-1708283, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-1864505, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-1878971, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-1924314, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-1995426, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-2231712, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-2315034, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-2345153, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-2549377, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-2592344, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-2679354, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-2717612, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-2753855, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-2985470, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-3007478, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-3456737, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-3762694, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-3859358, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-5014931, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-6247632, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-6323253, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-6378370, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-6546423, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-6631413, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-7517396, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-7534372, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-7592454, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-7601857, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-7694653, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-7868593, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-8038161, http://linkedlifedata.com/resource/pubmed/commentcorrection/8655504-8451638
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
178
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3238-45
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
The Streptomyces peucetius drrC gene encodes a UvrA-like protein involved in daunorubicin resistance and production.
pubmed:affiliation
School of Pharmacy, University of Wisconsin, Madison, 53706, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't