Linked Life Data

8654983

Source:http://linkedlifedata.com/resource/pubmed/id/8654983

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-7-30
pubmed:databankReference
pubmed:abstractText
To determine the signal recognition particle (SRP)-SRP receptor (Srb) system in Bacillus subtilis (Bs), we cloned the Bs srb gene, which encodes a homologue of the mammalian SRP receptor alpha-subunit [Oguro et al., DNA Res. 2 (1995) 95-100]. We sequenced a 6098-bp DNA containing srb and analyzed the gene organization. Primer extension experiment and Northern blot analysis revealed that srb constitutes an operon with two additional ORFs. A database search of known proteins revealed that one encodes a homologue of Escherichia coli RNase III [36.0% identical amino acids (aa)] and the other encodes a homologue of yeast Smc1 (26.6% identical aa). We then constructed a Bs mutant in which srb expression was induced by IPTG. The depletion of Srb caused a defect in the cell growth and the cells became filamentous and twisted. Furthermore, pulse-chase experiments using this mutant revealed that the 17% of the beta-lactamase precursor accumulated in the cell after a 4-min chase in the absence of IPTG, although almost all of the precursors were converted into the mature from after a 1-min chase in the presence of IPTG.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
172
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
17-24
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
The effect of Srb, a homologue of the mammalian SRP receptor alpha-subunit, on Bacillus subtilis growth and protein translocation.
pubmed:affiliation
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't