Linked Life Data

8654909

Source:http://linkedlifedata.com/resource/pubmed/id/8654909

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1996-7-31
pubmed:databankReference
pubmed:abstractText
We report on microdissection, cloning and sequence, and Southern and fluorescence in situ hybridization (FISH) analysis of one moderately and one highly amplified repetitive DNA element, pHvMWG2314 and pHvMWG2315, respectively, isolated from barley (Hordeum vulgare L.) chromosome arm 3HL. The pHvMWG2315 sequence hybridizes to all 14 telomeric or subtelomeric regions of the barley chromosomes as determined by FISH. The 50 different hybridization sites that include intercalary signals allow the discrimination of all 14 chromosome arms and the construction of a kariotype of barley. The tandemly repeated subtelomeric element of 331 bp exists in all Triticeae species tested (H. vulgare, Agropyron elongatum, Secale cereale, Triticum tauschii, T. turgidum, and T. aestivum). It is AT rich (66%), exibits 84% sequence homology to subfragments of the D genome ¿specific¿ 1-kb element pAs1 of T. tauscii and 75% homology to interspersed genome-specific DNA sequence pHcKB6 from H. chilence. The repetitive sequence pHvMWG2314 is moderately amplified in barley and highly amplified in hexaploid wheat. The in situ experiments revealed no distinct signals on barley chromosomes, indicating a dispersed character for the sequence. The significance of the results for the identification of chromosomes and chromosome aberrations in FISH experiments are discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0831-2796
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1082-90
pubmed:dateRevised
2009-11-3
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Repeated DNA sequences isolated by microdissection. I. Karyotyping of barley (Hordeum vulgare L.).
pubmed:affiliation
Botanisches Institut, Ludwig-Maximilians-Universität München, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't