rdf:type |
|
lifeskim:mentions |
|
pubmed:issue |
12
|
pubmed:dateCreated |
1996-7-25
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/H48477,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/H48478,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/H48931,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/H48939,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/H52858,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/H54046,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/H54559,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/H54560,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/H56769,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/H56778,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/H64202,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/H64307
|
pubmed:abstractText |
A new and highly effective method, termed suppression subtractive hybridization (SSH), has been developed for the generation of subtracted cDNA libraries. It is based primarily on a recently described technique called suppression PCR and combines normalization and subtraction in a single procedure. The normalization step equalizes the abundance of cDNAs within the target population and the subtraction step excludes the common sequences between the target and driver populations. In a model system, the SSH technique enriched for rare sequences over 1,000-fold in one round of subtractive hybridization. We demonstrate its usefulness by generating a testis-specific cDNA library and by using the subtracted cDNA mixture as a hybridization probe to identify homologous sequences in a human Y chromosome cosmid library. The human DNA inserts in the isolated cosmids were further confirmed to be expressed in a testis-specific manner. These results suggest that the SSH technique is applicable to many molecular genetic and positional cloning studies for the identification of disease, developmental, tissue-specific, or other differentially expressed genes.
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-1284595,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-1354393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-1383934,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-1722336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-1766870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-2216762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-2307059,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-2339063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-2456582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-6199676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-6326111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-6688681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-7501479,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-7731798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-7838717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-8438152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8650213-8524670
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
|
pubmed:status |
MEDLINE
|
pubmed:month |
Jun
|
pubmed:issn |
0027-8424
|
pubmed:author |
pubmed-author:CampbellA PAP,
pubmed-author:ChenchikAA,
pubmed-author:DiatchenkoLL,
pubmed-author:GurskayaNN,
pubmed-author:HuangBB,
pubmed-author:LukyanovKK,
pubmed-author:LukyanovSS,
pubmed-author:MoqadamFF,
pubmed-author:RaoS LSL,
pubmed-author:SiebertP DPD,
pubmed-author:SverdlovE DED
|
pubmed:issnType |
Print
|
pubmed:day |
11
|
pubmed:volume |
93
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
6025-30
|
pubmed:dateRevised |
2009-11-18
|
pubmed:meshHeading |
pubmed-meshheading:8650213-Base Sequence,
pubmed-meshheading:8650213-Chromosome Mapping,
pubmed-meshheading:8650213-Cosmids,
pubmed-meshheading:8650213-DNA, Complementary,
pubmed-meshheading:8650213-DNA Probes,
pubmed-meshheading:8650213-Gene Expression Regulation,
pubmed-meshheading:8650213-Genomic Library,
pubmed-meshheading:8650213-Humans,
pubmed-meshheading:8650213-Molecular Sequence Data,
pubmed-meshheading:8650213-Nucleic Acid Hybridization,
pubmed-meshheading:8650213-Y Chromosome
|
pubmed:year |
1996
|
pubmed:articleTitle |
Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries.
|
pubmed:affiliation |
CLONTECH Laboratories, Inc., Palo Alto, CA 94303, USA.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|