|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
9
|
|
pubmed:dateCreated |
1996-7-25
|
|
pubmed:abstractText |
The early SV40 BstXI-BamHI (Bst/Bam) DNA fragment encodes exclusively for the second exon of the large T-antigen and contains the intact small t-antigen intron. Rat cells transformed by the p14T, a construct that carries the Bst/Bam DNA fragment as a tail-to-head tandem duplication, synthesize a truncated T-antigen (T1-antigen) without having a direct equivalent at the DNA level. Formation of the T1-mRNA occurs by means of two distinct mechanisms: alternative-tandem-cis-splicing and trans-splicing. To generate the T1-mRNA the cells utilize a cryptic 5' splice site, located within the second exon of the large T-antigen and the regular small t-antigen 3' splice site. Since these splice sites are in an inverted order two Bst/Bam transcripts are required to generate one T1-mRNA molecule. For alternative-tandem-cis-splicing the cells utilize a 4.4 kb pre-mRNA that contains the sequence of the entire Bst/Bam tandem repeat. The proximal Bst/Bam segment provides the 5' donor splice site and the distal segment the 3' acceptor site. This requires that the pre-mRNA not be cleaved after the RNA polymerase II has passed the polyadenylation signal of the proximal Bst/Bam DNA segment. Synthesis of the 4.4 kb pre-mRNA was demonstrable by RT-PCR but not by Northern blot analysis. For trans-splicing, the cells utilize two separate pre-mRNA molecules. One transcript provides the cryptic 5' splice donor site and the other the 3' splice acceptor site. To demonstrate this a three base pair deletion was introduced into the proximal Bst/Bam segment of the p14T DNA (p14Tdelta-3) as a marker, destroying the recognition site for Pf/MI restriction enzyme. This deletion allowed the differentiation between the proximal and distal Bst/Bam segment. RT-PCR analysis and DNA sequencing confirmed that the p14Tdelta-3 transformed cells generate the T1-mRNA by intra- and inter-molecular RNA splicing.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-1017010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-1353951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-1359430,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-1361868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-1386423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-16453785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-1833268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-1848665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-1902143,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-1915302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-1936995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-1948046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-1973075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-2142018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-2143583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-2163768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-2170025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-2208274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-2526683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-2839300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-3014439,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-3027668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-3594568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-3791410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-6096557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-6247631,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-6310338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-729775,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-7542587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-7732590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-7906237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-7926751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-8178363,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-8255783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-8257104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-8287476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-8384583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-8422973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-8440247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8649982-8458337
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
May
|
|
pubmed:issn |
0305-1048
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
24
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1653-61
|
|
pubmed:dateRevised |
2010-9-13
|
|
pubmed:meshHeading |
pubmed-meshheading:8649982-Alternative Splicing,
pubmed-meshheading:8649982-Animals,
pubmed-meshheading:8649982-Antigens, Polyomavirus Transforming,
pubmed-meshheading:8649982-Base Sequence,
pubmed-meshheading:8649982-Cell Line, Transformed,
pubmed-meshheading:8649982-Exons,
pubmed-meshheading:8649982-Molecular Sequence Data,
pubmed-meshheading:8649982-RNA, Messenger,
pubmed-meshheading:8649982-RNA Precursors,
pubmed-meshheading:8649982-RNA Splicing,
pubmed-meshheading:8649982-Rats,
pubmed-meshheading:8649982-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:8649982-Sequence Deletion,
pubmed-meshheading:8649982-Simian virus 40
|
|
pubmed:year |
1996
|
|
pubmed:articleTitle |
Trans-splicing and alternative-tandem-cis-splicing: two ways by which mammalian cells generate a truncated SV40 T-antigen.
|
|
pubmed:affiliation |
Institut für Molekularbiologie und Biochemie, Freie Universität, Berlin, Germany.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
