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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1996-7-25
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pubmed:abstractText |
Using the experimental system of simian virus 40 (SV40) pseudovirions we have previously shown that SV40 requires a specific DNA element for packaging, ses, which was mapped to the SV40 regulatory region. ses was previously found to play a role in facilitating the nucleosomal rearrangement required for chromatin condensation and viral packaging. Here, the fine structure of ses was investigated by genetic studies. Analyses of ses+ revertants indicated that in order to function, ses must be present in close proximity to the origin of replication (ori), supporting a role in the regulation of the viral life cycle. Fine dissection of ses was performed using a series of plasmids carrying mutations and deletions in this region. The results suggest that multiple DNA elements participate in the SV40 packaging process, including the GC-boxes and elements derived from the enhancer. The elements are redundant, and they can function in various combinations. Packaging efficiency correlated with the number of GC-boxes, known to bind Sp1. In addition, AP-2 binding elements appeared to more important than others. These findings were supported by experiments which showed that packaging was significantly enhanced by adding AP-2 binding sites to plasmids with large deletions and lacking those sites. The results imply that binding of Sp1 and/or AP-2 may participate in the packaging process.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Transposable Elements,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Regulatory and Accessory...,
http://linkedlifedata.com/resource/pubmed/chemical/activator protein-2 binding element
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0022-2836
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
259
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
69-80
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8648649-Animals,
pubmed-meshheading:8648649-Base Sequence,
pubmed-meshheading:8648649-Cells, Cultured,
pubmed-meshheading:8648649-Chromosome Mapping,
pubmed-meshheading:8648649-DNA, Viral,
pubmed-meshheading:8648649-DNA Transposable Elements,
pubmed-meshheading:8648649-DNA-Binding Proteins,
pubmed-meshheading:8648649-Haplorhini,
pubmed-meshheading:8648649-Kidney,
pubmed-meshheading:8648649-Models, Biological,
pubmed-meshheading:8648649-Molecular Sequence Data,
pubmed-meshheading:8648649-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:8648649-Sequence Deletion,
pubmed-meshheading:8648649-Simian virus 40,
pubmed-meshheading:8648649-Viral Regulatory and Accessory Proteins,
pubmed-meshheading:8648649-Virus Assembly
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pubmed:year |
1996
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pubmed:articleTitle |
The simian virus 40 packaging signal ses is composed of redundant DNA elements which are partly interchangeable.
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pubmed:affiliation |
Department of Hematology, Hebrew University Hadassah Medical School, Jerusalem, Israel.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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