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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1996-7-18
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pubmed:databankReference |
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pubmed:abstractText |
IX/X-bp is an anticoagulant protein isolated from the venom of the habu snake (Trimeresurus flavoviridis). It is a heterogeneous two-chain protein linked by an interchain S-S bond. We prepared a cDNA library from the venom gland of the habu snake in the vector pSPORT1. cDNA clones containing the coding sequences for IX/X-bp were isolated and sequenced to determine the structure of the proprotein of IX/X-bp. All cDNA clones containing coding sequences of either chain of IX/X-bp consisted of the 5'-end noncoding bases, the first ATG codon, a typical signal peptide sequence that was immediately followed by mature protein sequence that corresponded to one of the chains, a stop codon, the 3'-end noncoding bases, a polyadenylation signal, and a poly(A)+ region. These data indicate that the gene for each chain of the two-chain protein is transcribed and translated separately.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
220
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
382-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8645314-Amino Acid Sequence,
pubmed-meshheading:8645314-Anticoagulants,
pubmed-meshheading:8645314-Base Sequence,
pubmed-meshheading:8645314-Carrier Proteins,
pubmed-meshheading:8645314-Cloning, Molecular,
pubmed-meshheading:8645314-Codon,
pubmed-meshheading:8645314-Crotalid Venoms,
pubmed-meshheading:8645314-DNA, Complementary,
pubmed-meshheading:8645314-Disulfides,
pubmed-meshheading:8645314-Macromolecular Substances,
pubmed-meshheading:8645314-Molecular Sequence Data,
pubmed-meshheading:8645314-Reptilian Proteins,
pubmed-meshheading:8645314-Restriction Mapping,
pubmed-meshheading:8645314-Sequence Analysis, DNA
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pubmed:year |
1996
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pubmed:articleTitle |
cDNA cloning of IX/X-BP, a heterogeneous two-chain anticoagulant protein from snake venom.
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pubmed:affiliation |
Department of Biochemistry, Meiji College of Pharmacy, Yatocho, Tanashi, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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