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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1996-7-15
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pubmed:abstractText |
Cyclic ADP-ribose (cADPR), a well-known stimulator of ca(2+) release from the intracellular Ca(2+) pool, has recently emerged as a potential regulator of insulin secretion in pancreatic beta cells. As recently described, BST-1 is a glycosyl-phosphatidylinositol (GPI)-anchored surface molecule that exhibits homology with CD38 and Aplysia ADP-ribosyl cyclase. Like CD38, BST-1 has both ADP-ribosyl cyclase and cADPR hydrolase activities. As a step toward elucidating the physiological role of cADPR in insulin secretion we examined whether BST-1 is expressed in pancreatic islet cells. Sensitive reverse transcription-polymerase chain reaction detected almost as abundant expression of BST-1 mRNA in pancreatic islets as CD38 mRNA. Immunohistochemical analyses utilizing mirror image sections revealed that BST-1 protein is expressed in a majority of the cells in pancreatic islets and that at least beta cells and, to an even greater extent, alpha cells express BST-1. These observations suggest the involvement of multiple enzymes in the regulation of cADPR concentrations in pancreatic islet cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ADP-ribosyl Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/ADP-ribosyl cyclase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD38,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/CD38 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cd38 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/GPI-Linked Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glycosylphosphatidylinositols,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/N-Glycosyl Hydrolases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-291X
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pubmed:author |
pubmed-author:FujitaniYY,
pubmed-author:HanafusaTT,
pubmed-author:HiranoTT,
pubmed-author:IshiharaKK,
pubmed-author:ItohMM,
pubmed-author:KaishoTT,
pubmed-author:KajimotoYY,
pubmed-author:KamadaTT,
pubmed-author:MatsuokaTT,
pubmed-author:MatsuzawaYY,
pubmed-author:MiyagawaJJ,
pubmed-author:OkuyamaYY,
pubmed-author:WatadaHH,
pubmed-author:YamasakiYY,
pubmed-author:YoshidaHH
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
219
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
941-6
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:8645283-ADP-ribosyl Cyclase,
pubmed-meshheading:8645283-Animals,
pubmed-meshheading:8645283-Antigens, CD,
pubmed-meshheading:8645283-Antigens, CD38,
pubmed-meshheading:8645283-Antigens, Differentiation,
pubmed-meshheading:8645283-Antigens, Surface,
pubmed-meshheading:8645283-Aplysia,
pubmed-meshheading:8645283-Flow Cytometry,
pubmed-meshheading:8645283-GPI-Linked Proteins,
pubmed-meshheading:8645283-Gene Expression,
pubmed-meshheading:8645283-Glycosylphosphatidylinositols,
pubmed-meshheading:8645283-Humans,
pubmed-meshheading:8645283-Immunohistochemistry,
pubmed-meshheading:8645283-Islets of Langerhans,
pubmed-meshheading:8645283-Membrane Glycoproteins,
pubmed-meshheading:8645283-Mice,
pubmed-meshheading:8645283-Mice, Inbred BALB C,
pubmed-meshheading:8645283-N-Glycosyl Hydrolases,
pubmed-meshheading:8645283-Point Mutation,
pubmed-meshheading:8645283-Polymerase Chain Reaction,
pubmed-meshheading:8645283-RNA, Messenger,
pubmed-meshheading:8645283-Recombinant Proteins,
pubmed-meshheading:8645283-Sequence Homology, Amino Acid,
pubmed-meshheading:8645283-Transfection
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pubmed:year |
1996
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pubmed:articleTitle |
Pancreatic islet cells express BST-1, a CD38-like surface molecule having ADP-ribosyl cyclase activity.
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pubmed:affiliation |
First Department of Medicine, Osaka University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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