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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1996-7-17
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pubmed:abstractText |
The physical stability of pharmaceutical proteins in delivery environments is a critical determinant of biological potency and treatment efficacy, and yet it is often taken for granted. We studied both the bioactivity and physical stability of interleukin 2 upon delivery via continuous infusion. We found that the biological activity of the delivered protein was dramatically reduced by approximately 90% after a 24-hr infusion program. Only a portion of these losses could be attributed to direct protein deposition on the delivery surfaces. Analysis of delivered protein by size exclusion chromatography gave no indication of insulin-like, surface-induced aggregation phenomena. Examination of the secondary and tertiary structure of both adsorbed and delivered protein via Fourier-transform infrared spectroscopy, circular dichroism, and fluorescence spectroscopy indicated that transient surface association of interleukin 2 with the catheter tubing resulted in profound, irreversible structural changes that were responsible for the majority of the biological activity losses.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643597-1592178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643597-1946348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643597-2049386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643597-2370622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643597-2687836,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643597-2783421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643597-3264184,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643597-3395621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643597-3487260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643597-3488324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643597-3489989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643597-3493029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643597-3500515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643597-3790543,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643597-42450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643597-651665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643597-6572366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643597-7030122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643597-7764118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643597-8280492,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643597-8465960
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5460-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8643597-Acrylamide,
pubmed-meshheading:8643597-Acrylamides,
pubmed-meshheading:8643597-Adsorption,
pubmed-meshheading:8643597-Animals,
pubmed-meshheading:8643597-Biological Assay,
pubmed-meshheading:8643597-Cell Line,
pubmed-meshheading:8643597-Circular Dichroism,
pubmed-meshheading:8643597-Cloning, Molecular,
pubmed-meshheading:8643597-Drug Stability,
pubmed-meshheading:8643597-Escherichia coli,
pubmed-meshheading:8643597-Humans,
pubmed-meshheading:8643597-Infusion Pumps,
pubmed-meshheading:8643597-Infusions, Intravenous,
pubmed-meshheading:8643597-Interleukin-2,
pubmed-meshheading:8643597-Lymphocyte Activation,
pubmed-meshheading:8643597-Mice,
pubmed-meshheading:8643597-Potassium Iodide,
pubmed-meshheading:8643597-Protein Structure, Secondary,
pubmed-meshheading:8643597-Protein Structure, Tertiary,
pubmed-meshheading:8643597-Recombinant Proteins,
pubmed-meshheading:8643597-Spectroscopy, Fourier Transform Infrared,
pubmed-meshheading:8643597-Time Factors
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pubmed:year |
1996
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pubmed:articleTitle |
Irreversible inactivation of interleukin 2 in a pump-based delivery environment.
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pubmed:affiliation |
The Howard P. Isermann Department of Chemical Engineering, Applied Protein Biophysics Laboratory, Rensselaer Polytechnic Institute, Troy, NY 12180-3590, USA.
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pubmed:publicationType |
Journal Article
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