Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1996-7-17
pubmed:abstractText
The transcription factors c-myb and GATA-2 are both required for blood cell development in vivo and in vitro. However, very little is known on their mechanism(s) of action and whether they impact on complementary or overlapping pathways of hematopoietic proliferation and differentiation. We report here that embryonic stem (ES) cells transfected with c-myb or GATA-2 cDNAs, individually or in combination, underwent hematopoietic commitment and differentiation in the absence of added hematopoietic growth factors but that stimulation with c-kit and flt-3 ligands enhanced colony formation only in the c-myb transfectants. This enhancement correlated with c-kit and flt-3 surface receptor up-regulation in c-myb-(but not GATA-2-) transfected ES cells. Transfection of ES cells with either a c-myb or a GATA-2 antisense construct abrogated erythromyeloid colony-forming ability in methyl cellulose; however, introduction of a full-length GATA-2 or c-myb cDNA, respectively, rescued the hematopoiesis-deficient phenotype, although only c-myb-rescued ES cells expressed c-kit and flt-3 surface receptors and formed increased numbers of hematopoietic colonies upon stimulation with the cognate ligands. These results are in agreement with previous studies indicating a fundamental role of c-myb and GATA-2 in hematopoiesis. Of greater importance, our studies suggest that GATA-2 and c-myb exert their roles in hematopoietic gene regulation through distinct mechanisms of action in nonoverlapping pathways.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643572-1370637, http://linkedlifedata.com/resource/pubmed/commentcorrection/8643572-1707696, http://linkedlifedata.com/resource/pubmed/commentcorrection/8643572-1709592, http://linkedlifedata.com/resource/pubmed/commentcorrection/8643572-1987478, http://linkedlifedata.com/resource/pubmed/commentcorrection/8643572-2440339, http://linkedlifedata.com/resource/pubmed/commentcorrection/8643572-3010282, http://linkedlifedata.com/resource/pubmed/commentcorrection/8643572-3897439, http://linkedlifedata.com/resource/pubmed/commentcorrection/8643572-7505204, http://linkedlifedata.com/resource/pubmed/commentcorrection/8643572-7519070, http://linkedlifedata.com/resource/pubmed/commentcorrection/8643572-7565760, http://linkedlifedata.com/resource/pubmed/commentcorrection/8643572-7790393, http://linkedlifedata.com/resource/pubmed/commentcorrection/8643572-7823931, http://linkedlifedata.com/resource/pubmed/commentcorrection/8643572-7830794, http://linkedlifedata.com/resource/pubmed/commentcorrection/8643572-7890597, http://linkedlifedata.com/resource/pubmed/commentcorrection/8643572-7926723, http://linkedlifedata.com/resource/pubmed/commentcorrection/8643572-8033210, http://linkedlifedata.com/resource/pubmed/commentcorrection/8643572-8078582, http://linkedlifedata.com/resource/pubmed/commentcorrection/8643572-8079170, http://linkedlifedata.com/resource/pubmed/commentcorrection/8643572-8630382
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34, http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/GATA2 Transcription Factor, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-kit, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myb, http://linkedlifedata.com/resource/pubmed/chemical/Receptor Protein-Tyrosine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/fms-Like Tyrosine Kinase 3
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
93
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5313-8
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:8643572-Animals, pubmed-meshheading:8643572-Antigens, CD34, pubmed-meshheading:8643572-Biological Markers, pubmed-meshheading:8643572-Cell Differentiation, pubmed-meshheading:8643572-Cell Line, pubmed-meshheading:8643572-DNA-Binding Proteins, pubmed-meshheading:8643572-GATA2 Transcription Factor, pubmed-meshheading:8643572-Hematopoiesis, pubmed-meshheading:8643572-Hematopoietic Stem Cells, pubmed-meshheading:8643572-Oncogenes, pubmed-meshheading:8643572-Polymerase Chain Reaction, pubmed-meshheading:8643572-Proto-Oncogene Proteins, pubmed-meshheading:8643572-Proto-Oncogene Proteins c-kit, pubmed-meshheading:8643572-Proto-Oncogene Proteins c-myb, pubmed-meshheading:8643572-Receptor Protein-Tyrosine Kinases, pubmed-meshheading:8643572-Recombinant Proteins, pubmed-meshheading:8643572-Trans-Activators, pubmed-meshheading:8643572-Transcription Factors, pubmed-meshheading:8643572-Transfection, pubmed-meshheading:8643572-fms-Like Tyrosine Kinase 3
pubmed:year
1996
pubmed:articleTitle
The transcription factors c-myb and GATA-2 act independently in the regulation of normal hematopoiesis.
pubmed:affiliation
Department of Microbiology and Immunology, Kimmel Cancer Institute, Thomas Jefferson University, Philadelphia, PA 19107, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't