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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1996-7-18
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pubmed:abstractText |
Interaction between CD40 on B cells and CD40 ligand molecules on T cells is pivotal for the generation of a thymus-dependent antibody response. Here we show that B cells deficient in CD40 expression are unable to elicit the proliferation of allogeneic T cells in vitro. More importantly, mice immunized with CD40-/- B cells become tolerant to allogeneic major histocompatibility complex (MHC) antigens as measured by a mixed lymphocyte reaction and cytotoxic T-cell assay. The failure of CD40-/- B cells to serve as antigen presenting cells in vitro was corrected by the addition of anti-CD28 mAb. Moreover, lipopolysaccharide stimulation, which upregulates B7 expression, reversed the inability of CD40-/- B cells to stimulate an alloresponse in vitro and abrogated the capacity of these B cells to induce tolerance in vivo. These results suggest that CD40 engagement by CD40 ligand expressed on antigen-activated T cells is critical for the upregulation of B7 molecules on antigen-presenting B cells that subsequently deliver the costimulatory signals necessary for T-cell proliferation and differentiation. Our experiments suggest a novel strategy for the induction of antigen-specific tolerance in vivo.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-1281209,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-1313950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-1385114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-1385153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-1701824,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-1714933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-1730913,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-1847724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-2113314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-2405272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-2964482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-2973060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-7504293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-7517359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-7522010,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-7524518,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-7527552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-7532456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-7534615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-7540943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-7681469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-7681471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-7681587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8643517-8184464
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD40,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD86,
http://linkedlifedata.com/resource/pubmed/chemical/CD40 Ligand,
http://linkedlifedata.com/resource/pubmed/chemical/Cd86 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Isoantigens,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4994-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8643517-Animals,
pubmed-meshheading:8643517-Antigens, CD,
pubmed-meshheading:8643517-Antigens, CD40,
pubmed-meshheading:8643517-Antigens, CD80,
pubmed-meshheading:8643517-Antigens, CD86,
pubmed-meshheading:8643517-B-Lymphocytes,
pubmed-meshheading:8643517-CD40 Ligand,
pubmed-meshheading:8643517-Immune Tolerance,
pubmed-meshheading:8643517-Isoantigens,
pubmed-meshheading:8643517-Ligands,
pubmed-meshheading:8643517-Lymphocyte Activation,
pubmed-meshheading:8643517-Lymphocyte Cooperation,
pubmed-meshheading:8643517-Lymphocyte Culture Test, Mixed,
pubmed-meshheading:8643517-Membrane Glycoproteins,
pubmed-meshheading:8643517-Mice,
pubmed-meshheading:8643517-Mice, Inbred BALB C,
pubmed-meshheading:8643517-Mice, Inbred C57BL,
pubmed-meshheading:8643517-Mice, Knockout,
pubmed-meshheading:8643517-T-Lymphocytes
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pubmed:year |
1996
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pubmed:articleTitle |
Induction of alloantigen-specific tolerance by B cells from CD40-deficient mice.
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pubmed:affiliation |
Division of Pediatric Oncology, Dana-Farber Cancer Institute, Havard Medical School, Boston, MA 02115, USA.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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