Linked Life Data

8643386

Source:http://linkedlifedata.com/resource/pubmed/id/8643386

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
33
pubmed:dateCreated
1996-7-17
pubmed:abstractText
Several distinct domains of U4 small nuclear (sn)RNA interact with other components of the spliceosome and are essential for pre-mRNA splicing. We have previously shown that single point mutations (Hu et al., 1995) in the central domain of the yeast U4 snRNA do not effect cell growth. In contrast to our results, this region has been reported to possess metal-binding or catalytic activity in vitro (Yang et al., 1994). In order to test if large mutations in the central domain effect cell growth and/or in vivo splicing, we have carried out further mutational analyses of this domain. A deletion of nucleotides from 62 to 88, including all those corresponding to the invariable positions of the hypothetical hammerhead, does not affect cell growth at/or above 25 degrees C. In addition, the central region (nucleotides 69-88) can be replaced by two copies of a 38-nt spacer inserted in the same orientation without any effect on cell growth or nuclear RNA splicing. These results suggest that the central domain of U4 snRNA functions primarily to separate the 5'- and 3'- domains of the snRNA by an optimal distance.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0261-3166
pubmed:author
pubmed:issnType
Print
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
25-8
pubmed:dateRevised
2010-4-28
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
The central region of the yeast U4 snRNA is not important for hammerhead catalysis, but may act as a domain spacer.
pubmed:affiliation
Department of Molecular and Medical Genetics, University of Toronto, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't