Linked Life Data

8643104

Source:http://linkedlifedata.com/resource/pubmed/id/8643104

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
17-18
pubmed:dateCreated
1996-7-15
pubmed:databankReference
pubmed:abstractText
An anti-DNA hybridoma derived from an MRL/lpr mouse secretes two different kappa light chains in combination with a single heavy chain. Multiple single cell clones express and secrete immunoglobulin containing both kappa light chains. The N-terminal protein sequences of the light chains correspond to sequences predicted from functionally rearranged mRNAs subjected to reverse transcription and amplified by polymerase chain reaction (PCR). Karyotype analysis of the hybridoma indicates a clonal line derived from the fusion of two cells. By amino acid sequence comparison and PCR analysis, both functional kappa light chains are derived from the MRL/lpr spleen. The two functional light chain cDNAs were cloned and co-transfected into COS-7 cells with the heavy chain cDNA. Only one of the light chains in combination with mAb 3E10 heavy chain confers anti-DNA reactivity. The presence of two separate kappa light chains and, therefore, two separate antigen receptors on a single B cell may have ramifications for both polyclonal activation and toleration of lupus B cells.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0161-5890
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1345-53
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1995
pubmed:articleTitle
Two kappa immunoglobulin light chains are secreted by an anti-DNA hybridoma: implications for isotypic exclusion.
pubmed:affiliation
Department of Medicine, Veteran's Affairs Medical Center, Sepulveda, CA 91343, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't