| pubmed-article:8642634 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8642634 | lifeskim:mentions | umls-concept:C0042776 | lld:lifeskim |
| pubmed-article:8642634 | lifeskim:mentions | umls-concept:C0011311 | lld:lifeskim |
| pubmed-article:8642634 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:8642634 | lifeskim:mentions | umls-concept:C0332307 | lld:lifeskim |
| pubmed-article:8642634 | lifeskim:mentions | umls-concept:C0026584 | lld:lifeskim |
| pubmed-article:8642634 | lifeskim:mentions | umls-concept:C0042720 | lld:lifeskim |
| pubmed-article:8642634 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:8642634 | lifeskim:mentions | umls-concept:C0079941 | lld:lifeskim |
| pubmed-article:8642634 | lifeskim:mentions | umls-concept:C0683598 | lld:lifeskim |
| pubmed-article:8642634 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:8642634 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:8642634 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:8642634 | pubmed:dateCreated | 1996-7-18 | lld:pubmed |
| pubmed-article:8642634 | pubmed:abstractText | The full-length premembrane (prM) coding region of the dengue virus type 2 (DEN-2; Jamaica) genome was expressed in C6/36 (Aedes albopictus) cells in either the sense or the antisense orientation from a double subgenomic Sindbis (dsSIN) virus. Northern (RNA) blot analysis confirmed the expression of sense or antisense DEN-2 prM RNA in infected C6/36 cells. PrM protein was demonstrated in cells infected with dsSIN virus expressing DEN-2 sense RNAs by an immunofluorescence assay. C6/36 cells were infected with each dsSIN virus at a multiplicity of infection (MOI) of 50 and challenged 48 h later with DEN-2 virus at an MOI of 0.1. Whereas C6/36 cells infected with a control of dsSIN virus supported high levels of DEN-2 replication, C6/36 cells infected with the dsSIN virus expressing prM antisense RNA were completely resistant to DEN-2 challenge. Cells expressing prM protein or untranslatable prM sense RNA also were resistant to DEN-2 challenge. Cells expressing prM protein demonstrated some breakthrough of DEN-2 virus when challenged at an MOI of 10. However, expressed untranslatable sense prM RNA conferred complete protection to challenge at the high MOI. | lld:pubmed |
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| pubmed-article:8642634 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8642634 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8642634 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8642634 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8642634 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:8642634 | pubmed:issn | 0022-538X | lld:pubmed |
| pubmed-article:8642634 | pubmed:author | pubmed-author:BlairC DCD | lld:pubmed |
| pubmed-article:8642634 | pubmed:author | pubmed-author:BeatyB JBJ | lld:pubmed |
| pubmed-article:8642634 | pubmed:author | pubmed-author:HiggsSS | lld:pubmed |
| pubmed-article:8642634 | pubmed:author | pubmed-author:OlsonK EKE | lld:pubmed |
| pubmed-article:8642634 | pubmed:author | pubmed-author:PowersA MAM | lld:pubmed |
| pubmed-article:8642634 | pubmed:author | pubmed-author:GainesP JPJ | lld:pubmed |
| pubmed-article:8642634 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8642634 | pubmed:volume | 70 | lld:pubmed |
| pubmed-article:8642634 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8642634 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8642634 | pubmed:pagination | 2132-7 | lld:pubmed |
| pubmed-article:8642634 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:8642634 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8642634 | pubmed:articleTitle | Pathogen-derived resistance to dengue type 2 virus in mosquito cells by expression of the premembrane coding region of the viral genome. | lld:pubmed |
| pubmed-article:8642634 | pubmed:affiliation | Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523, USA. | lld:pubmed |
| pubmed-article:8642634 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8642634 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8642634 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:1494449 | entrezgene:pubmed | pubmed-article:8642634 | lld:entrezgene |
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