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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0011311,
umls-concept:C0017262,
umls-concept:C0026584,
umls-concept:C0042720,
umls-concept:C0042776,
umls-concept:C0079941,
umls-concept:C0185117,
umls-concept:C0332307,
umls-concept:C0683598,
umls-concept:C2911684
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pubmed:issue |
4
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pubmed:dateCreated |
1996-7-18
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pubmed:abstractText |
The full-length premembrane (prM) coding region of the dengue virus type 2 (DEN-2; Jamaica) genome was expressed in C6/36 (Aedes albopictus) cells in either the sense or the antisense orientation from a double subgenomic Sindbis (dsSIN) virus. Northern (RNA) blot analysis confirmed the expression of sense or antisense DEN-2 prM RNA in infected C6/36 cells. PrM protein was demonstrated in cells infected with dsSIN virus expressing DEN-2 sense RNAs by an immunofluorescence assay. C6/36 cells were infected with each dsSIN virus at a multiplicity of infection (MOI) of 50 and challenged 48 h later with DEN-2 virus at an MOI of 0.1. Whereas C6/36 cells infected with a control of dsSIN virus supported high levels of DEN-2 replication, C6/36 cells infected with the dsSIN virus expressing prM antisense RNA were completely resistant to DEN-2 challenge. Cells expressing prM protein or untranslatable prM sense RNA also were resistant to DEN-2 challenge. Cells expressing prM protein demonstrated some breakthrough of DEN-2 virus when challenged at an MOI of 10. However, expressed untranslatable sense prM RNA conferred complete protection to challenge at the high MOI.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-1340158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-1368791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-1372987,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-15463283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-15463689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-1641986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-2020552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-2174669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-2345967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-2410549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-2440339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-2827375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-3024394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-3277268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-3457472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-3479621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-6285749,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-7142968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-7551198,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-7983769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-8030366,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-8111422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-8146129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-8388278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-8411360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-8429301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8642634-8903160
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
70
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2132-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8642634-Aedes,
pubmed-meshheading:8642634-Animals,
pubmed-meshheading:8642634-Base Sequence,
pubmed-meshheading:8642634-Cell Line,
pubmed-meshheading:8642634-Cricetinae,
pubmed-meshheading:8642634-DNA, Viral,
pubmed-meshheading:8642634-Dengue Virus,
pubmed-meshheading:8642634-Gene Expression,
pubmed-meshheading:8642634-Genetic Engineering,
pubmed-meshheading:8642634-Genome, Viral,
pubmed-meshheading:8642634-Humans,
pubmed-meshheading:8642634-Molecular Sequence Data,
pubmed-meshheading:8642634-RNA, Antisense,
pubmed-meshheading:8642634-RNA, Viral,
pubmed-meshheading:8642634-Sindbis Virus,
pubmed-meshheading:8642634-Viral Envelope Proteins
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pubmed:year |
1996
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pubmed:articleTitle |
Pathogen-derived resistance to dengue type 2 virus in mosquito cells by expression of the premembrane coding region of the viral genome.
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pubmed:affiliation |
Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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