Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1996-7-17
|
| pubmed:abstractText |
We have investigated the expression of CD41a (gpIIbIIIa) on a subpopulation of human fetal bone marrow (FBM) CD34+ progenitor cells. Human FBM CD34+Lin- cells were subfractionated into CD41a+ and CD41a- subpopulations by flow cytometry. All the megakaryocyte colony-forming cells (CFU-MK) and almost all the burst-forming units-megakaryocyte (BFU-MK) were found within the CD41a+ subpopulation. In addition, a 14-fold greater number of granulocyte-macrophage colony-forming units (CFU-GM) and a five-fold greater number of mixed lineage progenitor cells (CFU-mix) were observed within the CD34+Lin-CD41a+ subpopulation compared to the CD34+Lin-CD41a- subpopulation. The high proliferative potential of CD34+Lin-CD41a+ cells was demonstrated by their capacity to expand in in vitro culture containing human plasma and recombinant Mpl ligand (thrombopoietin [Tpo]) with production of over 80% CD41b+ (gpIIb+) MKs. However, in long-term bone marrow cultures, the CD34+Lin-CD41a- population contained a significantly higher frequency of cobblestone area-forming cells (CAFC) than the CD34+Lin-CD41a+ population, indicating the presence of a primitive hematopoietic stem cell (HSC) population within the CD34+Lin-CD41a- subset. These data suggest that fetal CD34+Lin-CD41a+ cells are enriched for MK progenitor cells (CFU-MK and BFU-MK), myeloid progenitors, and CFU-mix but do not contain the more primitive CAFC.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0301-472X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
24
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
236-45
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8641347-Antigens, CD34,
pubmed-meshheading:8641347-Bone Marrow,
pubmed-meshheading:8641347-Bone Marrow Cells,
pubmed-meshheading:8641347-Cell Differentiation,
pubmed-meshheading:8641347-Cell Lineage,
pubmed-meshheading:8641347-Cell Separation,
pubmed-meshheading:8641347-Cells, Cultured,
pubmed-meshheading:8641347-Colony-Forming Units Assay,
pubmed-meshheading:8641347-Flow Cytometry,
pubmed-meshheading:8641347-Hematopoietic Stem Cells,
pubmed-meshheading:8641347-Humans,
pubmed-meshheading:8641347-Immunophenotyping,
pubmed-meshheading:8641347-Megakaryocytes,
pubmed-meshheading:8641347-Platelet Glycoprotein GPIIb-IIIa Complex,
pubmed-meshheading:8641347-Stem Cells,
pubmed-meshheading:8641347-Thrombopoietin
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Fetal bone marrow CD34+CD41+ cells are enriched for multipotent hematopoietic progenitors, but not for pluripotent stem cells.
|
| pubmed:affiliation |
SyStemix Inc., Palo Alto, CA 94304, USA.
|
| pubmed:publicationType |
Journal Article
|