Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1996-7-18
pubmed:abstractText
Transcriptional repression at the silent yeast mating type loci is achieved through the formation of a particular nucleoprotein complex at specific cis-acting elements called silencers. This complex in turn appears to initiate the spreading of a histone binding protein complex into the surrounding chromatin, which restricts accessibility of the region to the transcription machinery. We have investigated long-range, cooperative effects between silencers by studying the repression of a reporter gene integrated at the HML locus flanked by various combinations of wild-type and mutated silencer sequences. Two silencers can cooperate over >4000 bp to repress transcription efficiently. More importantly, a single binding site for either the repressor activator protein 1 (Rap1), the autonomous replicating sequence (ARS) binding factor 1 (Abf1) or the origin recognition complex (ORC) can enhance the action of a distant silencer without acting as a silencer on its own. Functional cooperativity is demonstrated using a quantitative assay for repression, and varies with the affinity of the binding sites used. Since the repression mechanism is Sir dependent, the Rap1, ORC and/or Abf1 proteins bound to distant DNA elements may interact to create an interface of sufficiently high affinity such that Sir-containing complexes bind, nucleating the silent chromatin state.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-1322207, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-14731767, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-1480108, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-1577274, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-1737616, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-1913809, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-1915278, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-1922068, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-1945859, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-1946372, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-1964349, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-1990267, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-2005909, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-2010086, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-2015622, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-2120676, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-2197599, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-2201690, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-2548735, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-2655930, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-2659436, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-2667765, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-2689860, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-2843294, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-2998940, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-3023863, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-3046937, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-3048701, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-3146523, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-3275867, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-3315230, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-3938367, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-6092645, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-6301690, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-6312261, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-6379190, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-7545955, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-7580138, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-7613086, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-7744964, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-7774809, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-7791756, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-7809947, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-7867066, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-7867933, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-7896088, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-7917328, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-7926741, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-7958893, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-8032206, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-8183346, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-8206954, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-8209257, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-8221892, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-8221893, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-8264612, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-8266071, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-8266072, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-8319906, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-8319907, http://linkedlifedata.com/resource/pubmed/commentcorrection/8641284-8510148
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins, http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Histone Deacetylases, http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides, http://linkedlifedata.com/resource/pubmed/chemical/SIR2 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Silent Information Regulator..., http://linkedlifedata.com/resource/pubmed/chemical/Sirtuin 2, http://linkedlifedata.com/resource/pubmed/chemical/Sirtuins, http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators, http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase, http://linkedlifedata.com/resource/pubmed/chemical/rap GTP-Binding Proteins
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0261-4189
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2184-95
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