Linked Life Data

8640931

Source:http://linkedlifedata.com/resource/pubmed/id/8640931

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1996-7-17
pubmed:abstractText
The present study investigated the activity and cellular localization of individual isoenzymes of nitric oxide synthase (NOS) using immunohistochemistry and the [3H]citrulline assay in normal colorectal epithelia and neoplastic tissue. Intracellular localization of isoenzymes of NOS was detectable by immunohistochemistry in normal epithelial cells. Colorectal adenocarcinomas had a marked reduction of both Inducible NOS (iNOS) and constitutive NOS (cNOS) expression. Expression of iNOS was completely absent in tumour cells (P < 0.0001), while cNOS was reduced in 66% and absent in 34% of tumours studied when compared with controls (P < 0.0001). NOS activity using the [3H]citrulline assay was detectable in normal epithelium and was found to be reduced in tumours (P < 0.001). In addition, colonic adenomas had reduced INOS but not cNOS expression when compared with controls (P < 0.003 and P = 0.39 respectively). We conclude that NOS is present and active within the epithelium of the normal colon, with localization of the individual isoenzymes. Furthermore, there was loss of activity and expression of individual isoenzymes in colonic neoplasms.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0143-3334
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1171-4
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Nitric oxide synthase activity and expression in human colorectal cancer.
pubmed:affiliation
Department of Surgery, National University of Singapore.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't