Linked Life Data

8640830

Source:http://linkedlifedata.com/resource/pubmed/id/8640830

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1996-7-15
pubmed:abstractText
To elucidate the role of cyclin E in cell growth and tumorigenesis in mammary epithelial cells, we have used retrovirus-mediated transduction to generate derivatives of the nontransformed HC11 mouse mammary epithelial cell line that stably express a human cyclin E cDNA (HU4). These derivatives expressed two distinct forms of the exogenous cyclin E protein, which were about M(r) 50,000 and M(r) 42,000, thus corresponding to endogenous cyclin E proteins found in human cells. In contrast to results obtained previously in fibroblasts, overexpression of the HU4 cyclin E cDNA in HC11 cells was associated with an increase in cell size, lengthening of G(1), and inhibition of both anchorage-dependent and independent growth. Furthermore, when quiescent serum-starved cells were restimulated with serum, entry into the S-phase was delayed in the overexpressor cells. Under these conditions, there was also delayed induction in the expression of the endogenous cyclin E protein and in other events involved in the G(1) transition. Despite the high level of expression of the exogenous cyclin E, the derivatives did not display increased cyclin E-associated in vitro kinase activity. The HC11 cells that overexpressed the exogenous cyclin E displayed an increase in the cyclin/cyclin-dependent kinase inhibitor p27(Kip1) in both asynchronous exponentially dividing and synchronous cell populations. These findings indicate that increased expression of this cyclin E cDNA in HC11 cells inhibits rather than stimulates growth and that this may be due to increased expression of the inhibitor p27(Kip1).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1389-99
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:8640830-Animals, pubmed-meshheading:8640830-Breast, pubmed-meshheading:8640830-Breast Neoplasms, pubmed-meshheading:8640830-Caseins, pubmed-meshheading:8640830-Cell Cycle, pubmed-meshheading:8640830-Cell Cycle Proteins, pubmed-meshheading:8640830-Cell Line, Transformed, pubmed-meshheading:8640830-Cyclin-Dependent Kinase Inhibitor p27, pubmed-meshheading:8640830-Cyclin-Dependent Kinases, pubmed-meshheading:8640830-Cyclins, pubmed-meshheading:8640830-DNA, Complementary, pubmed-meshheading:8640830-Female, pubmed-meshheading:8640830-Fibroblasts, pubmed-meshheading:8640830-G0 Phase, pubmed-meshheading:8640830-G1 Phase, pubmed-meshheading:8640830-G2 Phase, pubmed-meshheading:8640830-Gene Expression, pubmed-meshheading:8640830-Humans, pubmed-meshheading:8640830-Mammary Glands, Animal, pubmed-meshheading:8640830-Mice, pubmed-meshheading:8640830-Mice, Inbred BALB C, pubmed-meshheading:8640830-Microtubule-Associated Proteins, pubmed-meshheading:8640830-Molecular Weight, pubmed-meshheading:8640830-RNA, Messenger, pubmed-meshheading:8640830-S Phase, pubmed-meshheading:8640830-Tumor Cells, Cultured, pubmed-meshheading:8640830-Tumor Suppressor Proteins
pubmed:year
1996
pubmed:articleTitle
Overexpression of cyclin E in the HC11 mouse mammary epithelial cell line is associated with growth inhibition and increased expression of p27(Kip1).
pubmed:affiliation
Columbia-Presbyterian Cancer Center, Columbia University, New York 10032, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't