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pubmed-article:8640554pubmed:abstractTextIn mammalian cells, genetic instructions are usually revised by RNA splicing before they are translated to proteins. Here we demonstrate that a trans-splicing group I ribozyme can be employed to intentionally modify the sequence of targeted transcripts in tissue culture cells. By analyzing the ribozyme reaction products, we demonstrate that targeted trans-splicing can proceed in murine fibroblasts with high fidelity, providing direct evidence that ribozymes function as anticipated in a therapeutically relevant setting. Trans-splicing is not very specific however, and the ribozyme reacted with and tagged a variety of cellular transcripts with its 3' exon sequence. RNA tagging provides a unique approach to study RNA catalysis in mammalian cells. Such analysis should facilitate the logical development of safe, therapeutic ribozymes that can repair mutant RNAs associated with a variety of inherited diseases.lld:pubmed
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pubmed-article:8640554pubmed:articleTitleTagging ribozyme reaction sites to follow trans-splicing in mammalian cells.lld:pubmed
pubmed-article:8640554pubmed:affiliationDepartment of Experimental Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA.lld:pubmed
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pubmed-article:8640554pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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