pubmed-article:8640554 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:8640554 | lifeskim:mentions | umls-concept:C0205145 | lld:lifeskim |
pubmed-article:8640554 | lifeskim:mentions | umls-concept:C1512977 | lld:lifeskim |
pubmed-article:8640554 | lifeskim:mentions | umls-concept:C0080125 | lld:lifeskim |
pubmed-article:8640554 | lifeskim:mentions | umls-concept:C0443286 | lld:lifeskim |
pubmed-article:8640554 | lifeskim:mentions | umls-concept:C0600448 | lld:lifeskim |
pubmed-article:8640554 | lifeskim:mentions | umls-concept:C1719822 | lld:lifeskim |
pubmed-article:8640554 | pubmed:issue | 6 | lld:pubmed |
pubmed-article:8640554 | pubmed:dateCreated | 1996-7-15 | lld:pubmed |
pubmed-article:8640554 | pubmed:abstractText | In mammalian cells, genetic instructions are usually revised by RNA splicing before they are translated to proteins. Here we demonstrate that a trans-splicing group I ribozyme can be employed to intentionally modify the sequence of targeted transcripts in tissue culture cells. By analyzing the ribozyme reaction products, we demonstrate that targeted trans-splicing can proceed in murine fibroblasts with high fidelity, providing direct evidence that ribozymes function as anticipated in a therapeutically relevant setting. Trans-splicing is not very specific however, and the ribozyme reacted with and tagged a variety of cellular transcripts with its 3' exon sequence. RNA tagging provides a unique approach to study RNA catalysis in mammalian cells. Such analysis should facilitate the logical development of safe, therapeutic ribozymes that can repair mutant RNAs associated with a variety of inherited diseases. | lld:pubmed |
pubmed-article:8640554 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8640554 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8640554 | pubmed:language | eng | lld:pubmed |
pubmed-article:8640554 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8640554 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:8640554 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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pubmed-article:8640554 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:8640554 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:8640554 | pubmed:month | Jun | lld:pubmed |
pubmed-article:8640554 | pubmed:issn | 1078-8956 | lld:pubmed |
pubmed-article:8640554 | pubmed:author | pubmed-author:LeeS WSW | lld:pubmed |
pubmed-article:8640554 | pubmed:author | pubmed-author:JonesJ TJT | lld:pubmed |
pubmed-article:8640554 | pubmed:author | pubmed-author:SullengerB... | lld:pubmed |
pubmed-article:8640554 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:8640554 | pubmed:volume | 2 | lld:pubmed |
pubmed-article:8640554 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:8640554 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:8640554 | pubmed:pagination | 643-8 | lld:pubmed |
pubmed-article:8640554 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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pubmed-article:8640554 | pubmed:year | 1996 | lld:pubmed |
pubmed-article:8640554 | pubmed:articleTitle | Tagging ribozyme reaction sites to follow trans-splicing in mammalian cells. | lld:pubmed |
pubmed-article:8640554 | pubmed:affiliation | Department of Experimental Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA. | lld:pubmed |
pubmed-article:8640554 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:8640554 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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