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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1996-7-15
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pubmed:abstractText |
In mammalian cells, genetic instructions are usually revised by RNA splicing before they are translated to proteins. Here we demonstrate that a trans-splicing group I ribozyme can be employed to intentionally modify the sequence of targeted transcripts in tissue culture cells. By analyzing the ribozyme reaction products, we demonstrate that targeted trans-splicing can proceed in murine fibroblasts with high fidelity, providing direct evidence that ribozymes function as anticipated in a therapeutically relevant setting. Trans-splicing is not very specific however, and the ribozyme reacted with and tagged a variety of cellular transcripts with its 3' exon sequence. RNA tagging provides a unique approach to study RNA catalysis in mammalian cells. Such analysis should facilitate the logical development of safe, therapeutic ribozymes that can repair mutant RNAs associated with a variety of inherited diseases.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1078-8956
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
643-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8640554-3T3 Cells,
pubmed-meshheading:8640554-Animals,
pubmed-meshheading:8640554-Base Sequence,
pubmed-meshheading:8640554-Cytoplasm,
pubmed-meshheading:8640554-DNA-Directed RNA Polymerases,
pubmed-meshheading:8640554-Lac Operon,
pubmed-meshheading:8640554-Mice,
pubmed-meshheading:8640554-Models, Genetic,
pubmed-meshheading:8640554-Molecular Sequence Data,
pubmed-meshheading:8640554-Plasmids,
pubmed-meshheading:8640554-Polymerase Chain Reaction,
pubmed-meshheading:8640554-RNA,
pubmed-meshheading:8640554-RNA, Bacterial,
pubmed-meshheading:8640554-RNA, Catalytic,
pubmed-meshheading:8640554-RNA Splicing,
pubmed-meshheading:8640554-Substrate Specificity,
pubmed-meshheading:8640554-Tetrahymena thermophila,
pubmed-meshheading:8640554-Transcription, Genetic,
pubmed-meshheading:8640554-Transfection,
pubmed-meshheading:8640554-Viral Proteins
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pubmed:year |
1996
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pubmed:articleTitle |
Tagging ribozyme reaction sites to follow trans-splicing in mammalian cells.
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pubmed:affiliation |
Department of Experimental Surgery, Duke University Medical Center, Durham, North Carolina 27710, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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