Linked Life Data

8639696

Source:http://linkedlifedata.com/resource/pubmed/id/8639696

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-7-17
pubmed:abstractText
The thymic microenvironment is under intrinsic and extrinsic control circuits by several elements including hormones, neuropeptides, lymphokines, innervation and cell contact. P2 purinergic receptors have been described in a number of cells including macrophages, thymocytes, and other cells of the immune-inflammatory system. Here, we use the whole-cell patch-clamp technique and dye permeabilization assays to investigate the presence of ionic channels and purinergic receptors in one microenvironmental thymic component, namely the phagocytic cell of the thymic reticulum. At holding potentials ranging from -30 to -60 mV, applications of extracellular ATP in the vicinity of the cell membrane induce a transient and fast-activating inward current followed in most cells by an outward current. The whole event lasts 5-20 s. The inward current has a reversal potential close to 0 mV and the outward current can be ascribed to a Ca2+ -dependent K+ conductance. Both currents are inhibited by Mg2+, suggesting that the phenomenon is mediated by ATP4-. ATP-gamma-S can also induce both inward and outward currents. Exposure of phagocytic cells of the thymic reticulum to 5 mM ATP for 10 min induced permeabilization to lucifer yellow but not to the larger dyes trypan blue and rhodamine-dextran, suggesting a molecular weight cut-off smaller than 900. These observations lead us to conclude that phagocytic cells of the thymic reticulum express P2Z purinergic receptors that can mobilize Ca2+, induce the opening of ionic channels and permeabilize the cell membrane.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-3002
pubmed:author
pubmed:issnType
Print
pubmed:day
26
pubmed:volume
1280
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
217-22
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Characterization of P2Z purinergic receptors on phagocytic cells of the thymic reticulum in culture.
pubmed:affiliation
Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brazil.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't