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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3 Pt 1
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pubmed:dateCreated |
1996-7-5
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pubmed:abstractText |
The role of P2 purinoceptor agonists in regulatory insulin secretion in Zucker diabetic fatty (ZDF) rats was studied using the isolated perfused pancreas and intracellular Ca2+ concentration ([Ca2+]i) microfluorimetry. The relative potency of different purinoceptor agonists to stimulate the insulin secretory process was consistent with the conclusion that responses in [Ca2+]i and insulin secretion are mediated by the P2y subtype of purinoceptors. Additional studies using specific antagonists of the Ca2+ signaling pathway indicated that activation of P2y purinoceptor releases Ca2+ from intracellular stores and promotes Ca2+ entry through voltage-independent rather than voltage-dependent Ca2+ channels on the beta-cell membrane. Perfused pancreas and isolated islets from ZDF rats demonstrated markedly reduced or absent insulin secretion and [Ca2+]i responses to glucose and KCl. In contrast, responses to P2y purinoceptor agonists were normal, indicating that the secretion coupling pathway activated by these agonists is preserved in glucose-unresponsive islets from diabetic animals. These observations raise the possibility that the purinoceptor pathway may play an important role in regulating insulin secretion in hyperinsulinemic non-insulin-dependent diabetes mellitus.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Diphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Monophosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Blood Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/FPL 66301,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Purinergic P2 Receptor Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Thionucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/adenosine 5'-O-(2-thiodiphosphate)
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0002-9513
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
270
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
E504-12
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8638699-Adenosine,
pubmed-meshheading:8638699-Adenosine Diphosphate,
pubmed-meshheading:8638699-Adenosine Monophosphate,
pubmed-meshheading:8638699-Adenosine Triphosphate,
pubmed-meshheading:8638699-Analysis of Variance,
pubmed-meshheading:8638699-Animals,
pubmed-meshheading:8638699-Blood Glucose,
pubmed-meshheading:8638699-Body Weight,
pubmed-meshheading:8638699-Calcium,
pubmed-meshheading:8638699-Diabetes Mellitus,
pubmed-meshheading:8638699-Diabetes Mellitus, Type 2,
pubmed-meshheading:8638699-Insulin,
pubmed-meshheading:8638699-Islets of Langerhans,
pubmed-meshheading:8638699-Kinetics,
pubmed-meshheading:8638699-Male,
pubmed-meshheading:8638699-Obesity,
pubmed-meshheading:8638699-Perfusion,
pubmed-meshheading:8638699-Potassium Chloride,
pubmed-meshheading:8638699-Purinergic P2 Receptor Agonists,
pubmed-meshheading:8638699-Rats,
pubmed-meshheading:8638699-Rats, Wistar,
pubmed-meshheading:8638699-Rats, Zucker,
pubmed-meshheading:8638699-Reference Values,
pubmed-meshheading:8638699-Thionucleotides,
pubmed-meshheading:8638699-Time Factors
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pubmed:year |
1996
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pubmed:articleTitle |
Preservation of insulin secretory responses to P2 purinoceptor agonists in Zucker diabetic fatty rats.
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pubmed:affiliation |
Department of Medicine, The University of Chicago, Illinois 06037, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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