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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3 Pt 1
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pubmed:dateCreated |
1996-7-5
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pubmed:abstractText |
The decreased osmotic fragility and reduced K+ content of BXD-31 mouse erythrocytes arise from variation at a single genetic locus. We compared ion transport in erythrocytes from BXD-31 mice and the parental strain, DBA/2J. The strains had similar rates for Na-K pump, Na/H exchange, Na-K-2Cl cotransport, Ca2+ activated K+ channel, or AE1-mediated SO4 transport. In contrast, K-Cl cotransport was twice as active in BXD-31 as in DBA/2J cells. Cl- dependent K+ efflux from BXD-31 cells displayed steep activation by acid pH (with maximal transport occurring at pH 6.75), whereas DBA/2J erythrocytes displayed a far less dramatic response to pH. Both strains displayed regulatory volume decrease in response to cell swelling. However, a 62% greater loss of cell K+ via K-Cl cotransport was observed in the BXD-31 strain. Furthermore the decreased osmotic fragility of BXD-31 red blood cells was normalized by treatment with nystatin to achieve normal cell K+ and water content. Thus upregulated K-Cl cotransport induces cell dehydration and K+ deficit in BXD-31 erythrocytes and causes their characteristic resistance to osmotic lysis.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4,4'-Diisothiocyanostilbene-2,2'-Dis...,
http://linkedlifedata.com/resource/pubmed/chemical/Bumetanide,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hypertonic Solutions,
http://linkedlifedata.com/resource/pubmed/chemical/Hypotonic Solutions,
http://linkedlifedata.com/resource/pubmed/chemical/Magnesium,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Potassium-Chloride Symporters,
http://linkedlifedata.com/resource/pubmed/chemical/Sulfates,
http://linkedlifedata.com/resource/pubmed/chemical/Symporters,
http://linkedlifedata.com/resource/pubmed/chemical/potassium-chloride symporters
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0002-9513
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
270
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
C866-77
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8638668-4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid,
pubmed-meshheading:8638668-Animals,
pubmed-meshheading:8638668-Bumetanide,
pubmed-meshheading:8638668-Carrier Proteins,
pubmed-meshheading:8638668-Crosses, Genetic,
pubmed-meshheading:8638668-Erythrocytes,
pubmed-meshheading:8638668-Hematocrit,
pubmed-meshheading:8638668-Hemolysis,
pubmed-meshheading:8638668-Hydrogen-Ion Concentration,
pubmed-meshheading:8638668-Hypertonic Solutions,
pubmed-meshheading:8638668-Hypotonic Solutions,
pubmed-meshheading:8638668-Magnesium,
pubmed-meshheading:8638668-Male,
pubmed-meshheading:8638668-Mice,
pubmed-meshheading:8638668-Mice, Inbred C57BL,
pubmed-meshheading:8638668-Mice, Inbred DBA,
pubmed-meshheading:8638668-Mice, Inbred Strains,
pubmed-meshheading:8638668-Potassium,
pubmed-meshheading:8638668-Sodium,
pubmed-meshheading:8638668-Sodium-Potassium-Chloride Symporters,
pubmed-meshheading:8638668-Species Specificity,
pubmed-meshheading:8638668-Sulfates,
pubmed-meshheading:8638668-Symporters
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pubmed:year |
1996
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pubmed:articleTitle |
Resistance to osmotic lysis in BXD-31 mouse erythrocytes: association with upregulated K-Cl cotransport.
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pubmed:affiliation |
Department of Laboratory Medicine, The Children's Hospital, Boston, Massachusetts 02115, USA.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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