Linked Life Data

8638659

Source:http://linkedlifedata.com/resource/pubmed/id/8638659

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3 Pt 1
pubmed:dateCreated
1996-7-5
pubmed:abstractText
The restitution of epithelial integrity is accomplished in part by cell migration. Studying this process, we have found that nitric oxide (NO) release migrating epithelial BSC-1 cells displayed a biphasic response to the inflicted wounds; an initial transient release of NO is followed by a delayed sustained elevation. Whereas the constitutive endothelial NO synthase (NOS) did not show any spatial or temporal changes associated with wounding, the inducible NOS became expressed 3 h after wounding and showed higher abundance at the edges of epithelial wounds. L-Arginine (L-Arg) or NO donor, S-nitroso-N-acetyl-DL-penicillamine, exerted motogenic effect in epithelial and endothelial cells. Inhibition of NOS with NG-nitro-L-arginine methyl ester (L-NAME) or a selective knockout of inducible NOS with antisense oligodeoxynucleotides reduced the rate of spontaneous or epidermal growth factor (EGF)-induced BSC-1 cell migration. Migrating cells showed the polarized expression of NOS, suggesting a head-to-rear NO gradient. Several growth factors (EGF, insulin-like growth factor I, hepatocyte growth factor, and fibroblast growth factor) were motogenic for BSC-1 cells, but this effect was abrogated by pretreatment with L-NAME. We conclude that endogenous NO production is a prerequisite for BSC-1 cell migration. A vectorial NO release may be essential for the spatially and temporally coordinated reciprocal phenomena that occur at the leading and trailing edge of locomoting epithelial cells. Although the exact mode of NO action remains uncertain, it is conceivable that the production of NO serves as a cellular switch from the stationary to the locomoting epithelial phenotype.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
270
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
C794-802
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8638659-Animals, pubmed-meshheading:8638659-Arginine, pubmed-meshheading:8638659-Base Sequence, pubmed-meshheading:8638659-Cell Line, pubmed-meshheading:8638659-Cercopithecus aethiops, pubmed-meshheading:8638659-Cyclic GMP, pubmed-meshheading:8638659-Dihydrolipoamide Dehydrogenase, pubmed-meshheading:8638659-Epithelial Cells, pubmed-meshheading:8638659-Epithelium, pubmed-meshheading:8638659-Histocytochemistry, pubmed-meshheading:8638659-Kidney Tubules, pubmed-meshheading:8638659-Microscopy, Fluorescence, pubmed-meshheading:8638659-Molecular Sequence Data, pubmed-meshheading:8638659-Movement, pubmed-meshheading:8638659-NG-Nitroarginine Methyl Ester, pubmed-meshheading:8638659-Nitric Oxide, pubmed-meshheading:8638659-Nitric Oxide Synthase, pubmed-meshheading:8638659-Oligonucleotides, Antisense, pubmed-meshheading:8638659-Penicillamine, pubmed-meshheading:8638659-Phenotype, pubmed-meshheading:8638659-S-Nitroso-N-Acetylpenicillamine, pubmed-meshheading:8638659-Thionucleotides
pubmed:year
1996
pubmed:articleTitle
Nitric oxide is necessary for a switch from stationary to locomoting phenotype in epithelial cells.
pubmed:affiliation
Department of Medicine, State University of New York at Stony Brook, Stony Brook, New York 11794, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't