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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1996-7-10
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/A61209,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/D16350,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L09598,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L15560,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/L17309,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M63968,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M84911,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M94729,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/M97217,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/P27550,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/S54801,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U24082,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X16989,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X16990,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X56211,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/X66425,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z46786,
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/Z66495
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pubmed:abstractText |
We determined the nucleotide (nt) sequence of the putative gene encoding acetyl-coenzyme A synthetase (ACS) from the parasitic protozoan Cryptosporidium parvum. The gene is single copy, located on a chromosome of approximately 1.08 mb, and has no introns. The gene is characterized by low codon usage bias and encodes a 694-amino acid (aa) protein with a predicted molecular size of 78 kDa, similar to other ACSs from different prokaryotic and eukaryotic species. Comparison of multiple protein alignments of ACSs revealed a new conserved sequence motif PKT(R/V/L)SGK(I/V/T)(T/M/V/K)R(R/N) near the C-terminus, which may be a signature for ACSs. This motif shares significant homology with sequences from other members of the AMP-binding family, has secondary structure similar to the purine-binding motif of ATP- and GTP-ases, and may play a role in the enzymatic activity of proteins from the AMP-binding family.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-3395
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
82
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
423-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8636846-Acetate-CoA Ligase,
pubmed-meshheading:8636846-Amino Acid Sequence,
pubmed-meshheading:8636846-Animals,
pubmed-meshheading:8636846-Base Sequence,
pubmed-meshheading:8636846-Blotting, Southern,
pubmed-meshheading:8636846-Codon,
pubmed-meshheading:8636846-Conserved Sequence,
pubmed-meshheading:8636846-Cryptosporidium parvum,
pubmed-meshheading:8636846-DNA, Protozoan,
pubmed-meshheading:8636846-Genes, Protozoan,
pubmed-meshheading:8636846-Molecular Sequence Data,
pubmed-meshheading:8636846-Molecular Weight,
pubmed-meshheading:8636846-Sequence Alignment,
pubmed-meshheading:8636846-Sequence Homology, Amino Acid
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pubmed:year |
1996
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pubmed:articleTitle |
The putative acetyl-CoA synthetase gene of Cryptosporidium parvum and a new conserved protein motif in acetyl-CoA synthetases.
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pubmed:affiliation |
Division of Biology, Kansas State University, Manhattan 66506, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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