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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1996-7-5
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pubmed:abstractText |
Cofilin is a low molecular weight actin-modulating protein whose structure and function are conserved among eucaryotes. Cofilin exhibits in vitro both a monomeric actin-sequestering activity and a filamentous actin-severing activity. To investigate in vivo functions of cofilin, cofilin was overexpressed in Dictyostelium discoideum cells. An increase in the content of D. discoideum cofilin (d-cofilin) by sevenfold induced a co-overproduction of actin by threefold. In cells over-expressing d-cofilin, the amount of filamentous actin but not that of monomeric actin was increased. Overexpressed d-cofilin co-sedimented with actin filaments, suggesting that the sequestering activity of d-cofilin is weak in vivo. The overexpression of d-cofilin increased actin bundles just beneath ruffling membranes where d-cofilin was co-localized. The overexpression of d-cofilin also stimulated cell movement as well as membrane ruffling. We have demonstrated in vitro that d-cofilin transformed latticework of actin filaments cross-linked by alpha-actinin into bundles probably by severing the filaments. D. discoideum cofilin may sever actin filaments in vivo and induce bundling of the filaments in the presence of cross-linking proteins so as to generate contractile systems involved in membrane ruffling and cell movement.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-1313794,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-1757465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-1757466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-3018510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-3030380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-3315240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-3320057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-3527055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-3941084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-4084546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-4254541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-6509022,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-7536193,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-7615564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-7738034,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-7755981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-7769003,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-8037689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-8139694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-8174642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-8234487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-8357799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-8399167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-8399168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-8421056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8636212-8440472
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9525
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
132
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
335-44
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8636212-Actin Depolymerizing Factors,
pubmed-meshheading:8636212-Actins,
pubmed-meshheading:8636212-Animals,
pubmed-meshheading:8636212-Base Sequence,
pubmed-meshheading:8636212-Cell Fractionation,
pubmed-meshheading:8636212-Cell Membrane,
pubmed-meshheading:8636212-Cell Movement,
pubmed-meshheading:8636212-DNA, Complementary,
pubmed-meshheading:8636212-DNA Primers,
pubmed-meshheading:8636212-Dictyostelium,
pubmed-meshheading:8636212-Gene Expression,
pubmed-meshheading:8636212-Microfilament Proteins,
pubmed-meshheading:8636212-Molecular Sequence Data,
pubmed-meshheading:8636212-Nerve Tissue Proteins,
pubmed-meshheading:8636212-Polymerase Chain Reaction,
pubmed-meshheading:8636212-Recombinant Proteins
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pubmed:year |
1996
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pubmed:articleTitle |
Overexpression of cofilin stimulates bundling of actin filaments, membrane ruffling, and cell movement in Dictyostelium.
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pubmed:affiliation |
Department of Cell Biology, Tokyo Metropolitan Institute of Medical Science, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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