Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1996-7-1
|
| pubmed:abstractText |
We have analysed the expression of five protein kinase C [PKC] isoforms in an in vitro model using nontumorigenic rat colonic epithelial cells FRC/TEX CL D [D/WT] and in the related tumorigenic Ha-ras-transformed FRC/TEX CL D/H-ras line [D/ras]. The PKC subspecies alpha, delta, epsilon and xi were expressed at the protein level in both D/WT and D/ras cells, while beta PKC was undetectable in both lines. The levels of expression of the delta and xi isoforms were similar in D/WT and D/ras cells. Alpha PKC expression was decreased and epsilon PKC was increased in D/ras cells compared to the D/WT line. To assess whether overexpression of epsilon PKC was linked to the transformed phenotype, we have generated from D/WT cells two clones (D/epsilon-5 and D/epsilon-9) which stably overexpress epsilon PKC about fivefold. Overexpression of epsilon PKC caused marked morphological changes in both transfected clones, which were accompanied by increased saturation densities and anchorage-independent colony formation in semisolid agar. These growth effects were attenuated or reversed by chronic incubation with phorbol 12-myristate 13-acetate. Furthermore, D/epsilon-5 and D/epsilon-9 cells formed tumors in athymic nude mice with 100% incidence while the parental D/WT or vector alone (D/MV12) controls produced no tumors. We conclude that epsilon PKC can act as an oncoprotein when modestly overproduced in nontumorigenic D/WT colonic cells, and that this isoform of PKC may be linked to ras-modulated signal transduction leading to neoplastic transformation in colonic epithelium.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0950-9232
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
12
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
847-54
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8632907-Animals,
pubmed-meshheading:8632907-Blotting, Western,
pubmed-meshheading:8632907-Cell Adhesion,
pubmed-meshheading:8632907-Cell Division,
pubmed-meshheading:8632907-Cell Line,
pubmed-meshheading:8632907-Cell Transformation, Neoplastic,
pubmed-meshheading:8632907-Colon,
pubmed-meshheading:8632907-Colonic Neoplasms,
pubmed-meshheading:8632907-Epithelial Cells,
pubmed-meshheading:8632907-Epithelium,
pubmed-meshheading:8632907-Gene Expression,
pubmed-meshheading:8632907-Genes, ras,
pubmed-meshheading:8632907-Isoenzymes,
pubmed-meshheading:8632907-Mice,
pubmed-meshheading:8632907-Mice, Nude,
pubmed-meshheading:8632907-Protein Kinase C,
pubmed-meshheading:8632907-Rats,
pubmed-meshheading:8632907-Recombinant Proteins,
pubmed-meshheading:8632907-Tetradecanoylphorbol Acetate,
pubmed-meshheading:8632907-Transfection,
pubmed-meshheading:8632907-Transplantation, Heterologous
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Overexpression of protein kinase C epsilon is oncogenic in rat colonic epithelial cells.
|
| pubmed:affiliation |
Istituto di Farmacologia, Università degli studi di Milano, Italy.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|