Linked Life Data

8632446

Source:http://linkedlifedata.com/resource/pubmed/id/8632446

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 1
pubmed:dateCreated
1996-7-1
pubmed:abstractText
This work describes the use of two cationic porphyrins for fluorescent DNA counterstaining of HeLa cells also stained by means of indirect immunofluorescence with fluorescein isothiocyanate (FITC)-conjugated secondary antibodies. Staining HeLa cells with meso-tetra(4-N-methylpyridyl)porphine (T4MPyP) and meso-tetra(p-N-trimethylanilinium)-porphine (TMAP), both used at 5 x 10(-6) M, gives rise to a deep red emission of chromatin from interphase nuclei and mitotic chromosomes when the cells are excited with blue (490 nm) light. The red-fluorescing chromatin contrasted very well with the yellowish-green emission from FITC-immunofluorescent staining. No significant difference in chromatin fluorescence found with either T4MPyP or TMAP was detected. Counterstaining with the porphyrins could be carried out as a separate step after immunolabeling or, more simply, by their inclusion in the mounting medium. Spectral analysis demonstrated that the fluorescent emission maximum of T4MPyP was at 665 nm and that the intensity of the fluorescent emission showed a considerable increase in the presence of DNA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0022-2720
pubmed:author
pubmed:issnType
Print
pubmed:volume
182
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
46-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Fluorescent porphyrin counterstaining of chromatin DNA in conjunction with immunofluorescence methods using FITC-labelled antibodies.
pubmed:affiliation
Departamento de Biología, Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't