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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
1996-7-3
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pubmed:abstractText |
The zeta-form 14-3-3 protein (14-3-3zeta) regulates protein kinases and interacts with several signaling molecules. We reported previously that a platelet adhesion receptor, glycoprotein (GP) Ib-IX, was associated with a 29-kDa protein with partial sequences identical to 14-3-3zeta. In this study, the interaction between GPIb-IX and recombinant 14-3-3zeta is reconstituted. Further, we show that the 14-3-3zeta binding site in GPIb is within a 15 residue sequence at the C terminus of GPIb-alpha, as indicated by antibody inhibition and direct binding of 14-3-3zeta to synthetic GPIb-alpha cytoplasmic domain peptides. The 14-3-3zeta binds to recombinant wild type GPIb-IX but not to the GPIb-alpha mutants lacking C-terminal 5 or more residues, suggesting that the C-terminal 5 residues of GPIb-alpha are critical. Similarity between the GPIb-alpha C-terminal sequence and the serine-rich regions of Raf and Bcr kinases suggests a possible serine-rich recognition motif for the 14-3-3 protein.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
271
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7362-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8631758-14-3-3 Proteins,
pubmed-meshheading:8631758-Amino Acid Sequence,
pubmed-meshheading:8631758-Animals,
pubmed-meshheading:8631758-Antibodies,
pubmed-meshheading:8631758-Base Sequence,
pubmed-meshheading:8631758-Binding Sites,
pubmed-meshheading:8631758-CHO Cells,
pubmed-meshheading:8631758-Cell Line,
pubmed-meshheading:8631758-Chromatography, Affinity,
pubmed-meshheading:8631758-Cloning, Molecular,
pubmed-meshheading:8631758-Cricetinae,
pubmed-meshheading:8631758-DNA Primers,
pubmed-meshheading:8631758-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8631758-Gene Library,
pubmed-meshheading:8631758-Humans,
pubmed-meshheading:8631758-Immunoblotting,
pubmed-meshheading:8631758-Kinetics,
pubmed-meshheading:8631758-Molecular Sequence Data,
pubmed-meshheading:8631758-Peptides,
pubmed-meshheading:8631758-Platelet Glycoprotein GPIb-IX Complex,
pubmed-meshheading:8631758-Polymerase Chain Reaction,
pubmed-meshheading:8631758-Protein Conformation,
pubmed-meshheading:8631758-Proteins,
pubmed-meshheading:8631758-Recombinant Proteins,
pubmed-meshheading:8631758-Sequence Homology, Amino Acid,
pubmed-meshheading:8631758-Serine,
pubmed-meshheading:8631758-Signal Transduction,
pubmed-meshheading:8631758-Transfection,
pubmed-meshheading:8631758-Tumor Cells, Cultured,
pubmed-meshheading:8631758-Tyrosine 3-Monooxygenase
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pubmed:year |
1996
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pubmed:articleTitle |
Identification of a binding sequence for the 14-3-3 protein within the cytoplasmic domain of the adhesion receptor, platelet glycoprotein Ib alpha.
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pubmed:affiliation |
Department of Vascular Biology, Scripps Research Institute, La Jolla, California 92037, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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