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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1996-7-3
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pubmed:abstractText |
Prespore and prestalk cells can be distinguished within aggregates of Dictyostelium by the expression of well-characterized cell type-specific genes. Fusion of the tagB regulatory region to Escherichia coli beta-galactosidase revealed that this prestalk specific gene marks the differentiation of the initial prestalk cell population, PST-1. The reporter gene was expressed normally in tagB- mutant cells despite the fact that they do not accumulate measurable levels of DIF-I, a morphogen that was previously implicated in prestalk differentiation. In an independent experimental system, wild-type cells respond to the addition of DIF-I by induction of the prestalk marker ecmA and repression of the prespore marker cotB. We found that DIF-1 did not affect the expression of the tagB or carB genes, both of which are prestalk specific and essential for PST-A cell differentiation. We conclude that the initiation of prestalk development is not dependent on DIF-1 and suggest that the morphogen participates mainly at later stages.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ATP-Binding Cassette Transporters,
http://linkedlifedata.com/resource/pubmed/chemical/Caenorhabditis elegans Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Differentiation-inducing factor-1...,
http://linkedlifedata.com/resource/pubmed/chemical/Helminth Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hexanones,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrocarbons, Chlorinated,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protozoan Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Serine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/TagB protein, Dictyostelium,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0012-1606
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
174
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
214-20
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8631494-ATP-Binding Cassette Transporters,
pubmed-meshheading:8631494-Animals,
pubmed-meshheading:8631494-Caenorhabditis elegans Proteins,
pubmed-meshheading:8631494-Carrier Proteins,
pubmed-meshheading:8631494-Cell Differentiation,
pubmed-meshheading:8631494-Cloning, Molecular,
pubmed-meshheading:8631494-Dictyostelium,
pubmed-meshheading:8631494-Escherichia coli,
pubmed-meshheading:8631494-Gene Expression Regulation, Developmental,
pubmed-meshheading:8631494-Genes, Reporter,
pubmed-meshheading:8631494-Helminth Proteins,
pubmed-meshheading:8631494-Hexanones,
pubmed-meshheading:8631494-Hydrocarbons, Chlorinated,
pubmed-meshheading:8631494-Proteins,
pubmed-meshheading:8631494-Protozoan Proteins,
pubmed-meshheading:8631494-Recombinant Fusion Proteins,
pubmed-meshheading:8631494-Serine Endopeptidases,
pubmed-meshheading:8631494-beta-Galactosidase
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pubmed:year |
1996
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pubmed:articleTitle |
Initial cell type divergence in Dictyostelium is independent of DIF-1.
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pubmed:affiliation |
Center for Molecular Genetics, Department of Biology, University of California, San Diego, La Jollo, California 92093, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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