Linked Life Data

8631160

Source:http://linkedlifedata.com/resource/pubmed/id/8631160

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1996-7-2
pubmed:abstractText
We have investigated the process by which the primitive erythroid cells develop during early vertebrate embryogenesis. Cultured Xenopus animal cap (AC) cells transiently activate the transcription of blood cell regulatory genes GATA-1 and GATA-2 but fail to commit stably to the blood lineage. By contrast, cells of the presumptive ventral marginal zone (VMZ), are committed by the midblastula transition (MBT) to express fully on erythroid program. Growth factor BMP-4, a member of the TGF-beta family of signaling molecules, has been implicated in the process of ventral mesoderm patterning. We show that expression of BMP-4 after MBT is sufficient to induce the blood program fully in AC cells. This includes high level expression of the blood markers SCL and globin, which are not activated in AC cells from uninjected embryos. Likewise, expression of a dominant negative receptor after MBT results in relatively normal embryos, which, however, completely lack differentiated blood cells. Our results are consistent with a role for BMP or BMP-like signaling during gastrulation in the differentiation of embryonic blood.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0192-253X
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
267-78
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
BMP-like signals are required after the midblastula transition for blood cell development.
pubmed:affiliation
Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't