rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1996-6-26
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pubmed:abstractText |
The solution conformations of the dinucleotide d(TT) and the modified duplex d(CGCGAATTCGCG)2 with N3'--> P5' phosphoramidate internucleoside linkages have been studied using circular dichroism (CD) and NMR spectroscopy. The CD spectra indicate that the duplex conformation is similar to that of isosequential phosphodiester RNA, a A-type helix, and is different from that of DNA, a B-type helix, NMR studies of model dimers d(TpT) and N3'--> P5' phosphoramidate d(TnpT) show that the sugar ring conformation changes from predominantly C2'-endo to C3'-endo when the 3'-phosphoester is replaced by a phosphoramidate group. Two-dimensional NMR (NOESY, DQF-COSY and TOCSY spectra) studies of the duplex provide additional details about the A-type duplex conformation of the oligonucleotide phosphoramidate and confirm that all furanose rings of 3'-aminonucleotides adopt predominantly N-type sugar puckering.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-1711677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-1854755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-1991117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-2350548,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-2410859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-2471547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-2856025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-6305651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-7071593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-7522550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-7826678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-8155979
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0305-1048
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
354-60
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
1996
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pubmed:articleTitle |
An oligodeoxyribonucleotide N3'--> P5' phosphoramidate duplex forms an A-type helix in solution.
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pubmed:affiliation |
Department of Chemistry, Georgia State University, Atlanta, 30303, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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