Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1996-6-26
pubmed:abstractText
The solution conformations of the dinucleotide d(TT) and the modified duplex d(CGCGAATTCGCG)2 with N3'--> P5' phosphoramidate internucleoside linkages have been studied using circular dichroism (CD) and NMR spectroscopy. The CD spectra indicate that the duplex conformation is similar to that of isosequential phosphodiester RNA, a A-type helix, and is different from that of DNA, a B-type helix, NMR studies of model dimers d(TpT) and N3'--> P5' phosphoramidate d(TnpT) show that the sugar ring conformation changes from predominantly C2'-endo to C3'-endo when the 3'-phosphoester is replaced by a phosphoramidate group. Two-dimensional NMR (NOESY, DQF-COSY and TOCSY spectra) studies of the duplex provide additional details about the A-type duplex conformation of the oligonucleotide phosphoramidate and confirm that all furanose rings of 3'-aminonucleotides adopt predominantly N-type sugar puckering.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-1711677, http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-1854755, http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-1991117, http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-2350548, http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-2410859, http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-2471547, http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-2856025, http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-6305651, http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-7071593, http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-7522550, http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-7826678, http://linkedlifedata.com/resource/pubmed/commentcorrection/8628662-8155979
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
354-60
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
An oligodeoxyribonucleotide N3'--> P5' phosphoramidate duplex forms an A-type helix in solution.
pubmed:affiliation
Department of Chemistry, Georgia State University, Atlanta, 30303, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't