| pubmed-article:8628661 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8628661 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:8628661 | lifeskim:mentions | umls-concept:C0012940 | lld:lifeskim |
| pubmed-article:8628661 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:8628661 | lifeskim:mentions | umls-concept:C1710082 | lld:lifeskim |
| pubmed-article:8628661 | lifeskim:mentions | umls-concept:C0016316 | lld:lifeskim |
| pubmed-article:8628661 | lifeskim:mentions | umls-concept:C1553165 | lld:lifeskim |
| pubmed-article:8628661 | lifeskim:mentions | umls-concept:C2349975 | lld:lifeskim |
| pubmed-article:8628661 | lifeskim:mentions | umls-concept:C1627358 | lld:lifeskim |
| pubmed-article:8628661 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:8628661 | pubmed:dateCreated | 1996-6-26 | lld:pubmed |
| pubmed-article:8628661 | pubmed:abstractText | Strand displacement amplification (9SDA) is an isothermal in vitro method of amplifying a DNA sequence prior to its detection. We have combined SDA with fluorescence polarization detection. A 5'-fluorescein-labelled oligodeoxynucleotide detector probe hybridizes to the amplification product that rises in concentration during SDA and the single- to double strand conversion is monitored through an increase in fluorescence polarization. Detection sensitivity can be enhanced by using a detector probe containing an EcoRI recognition sequence at its 5'-end that is not homologous to the target sequence. During SDA the probe is converted to a fully double-stranded form that specifically binds a genetically modified form of the endonuclease EcoRI which lacks cleavage activity but retains binding specificity. We have applied this SDA detection system to a target sequence specific for Mycobacterium tuberculosis. | lld:pubmed |
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| pubmed-article:8628661 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8628661 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8628661 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8628661 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8628661 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:8628661 | pubmed:issn | 0305-1048 | lld:pubmed |
| pubmed-article:8628661 | pubmed:author | pubmed-author:LinnC PCP | lld:pubmed |
| pubmed-article:8628661 | pubmed:author | pubmed-author:NadeauJ GJG | lld:pubmed |
| pubmed-article:8628661 | pubmed:author | pubmed-author:WalkerG TGT | lld:pubmed |
| pubmed-article:8628661 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8628661 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:8628661 | pubmed:volume | 24 | lld:pubmed |
| pubmed-article:8628661 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8628661 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8628661 | pubmed:pagination | 348-53 | lld:pubmed |
| pubmed-article:8628661 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:8628661 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8628661 | pubmed:articleTitle | DNA detection by strand displacement amplification and fluorescence polarization with signal enhancement using a DNA binding protein. | lld:pubmed |
| pubmed-article:8628661 | pubmed:affiliation | Becton Dickinson Research Center, Research Triangle Park, NC 27709-2016, USA. | lld:pubmed |
| pubmed-article:8628661 | pubmed:publicationType | Journal Article | lld:pubmed |
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