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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1996-6-26
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pubmed:abstractText |
Strand displacement amplification (9SDA) is an isothermal in vitro method of amplifying a DNA sequence prior to its detection. We have combined SDA with fluorescence polarization detection. A 5'-fluorescein-labelled oligodeoxynucleotide detector probe hybridizes to the amplification product that rises in concentration during SDA and the single- to double strand conversion is monitored through an increase in fluorescence polarization. Detection sensitivity can be enhanced by using a detector probe containing an EcoRI recognition sequence at its 5'-end that is not homologous to the target sequence. During SDA the probe is converted to a fully double-stranded form that specifically binds a genetically modified form of the endonuclease EcoRI which lacks cleavage activity but retains binding specificity. We have applied this SDA detection system to a target sequence specific for Mycobacterium tuberculosis.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628661-1579461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628661-1870966,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628661-2155396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628661-2291477,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628661-2745418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628661-2997157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628661-6273420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628661-8041630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628661-8220181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628661-8264674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628661-8347584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8628661-8633758
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonuclease EcoRI,
http://linkedlifedata.com/resource/pubmed/chemical/Escherichia coli Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescein,
http://linkedlifedata.com/resource/pubmed/chemical/Fluoresceins,
http://linkedlifedata.com/resource/pubmed/chemical/Gln-111 protein, E coli,
http://linkedlifedata.com/resource/pubmed/chemical/Molecular Probes
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
348-53
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8628661-Base Sequence,
pubmed-meshheading:8628661-DNA,
pubmed-meshheading:8628661-DNA, Bacterial,
pubmed-meshheading:8628661-DNA-Binding Proteins,
pubmed-meshheading:8628661-Deoxyribonuclease EcoRI,
pubmed-meshheading:8628661-Escherichia coli Proteins,
pubmed-meshheading:8628661-Fluorescein,
pubmed-meshheading:8628661-Fluoresceins,
pubmed-meshheading:8628661-Fluorescence Polarization,
pubmed-meshheading:8628661-Molecular Probes,
pubmed-meshheading:8628661-Molecular Sequence Data,
pubmed-meshheading:8628661-Mycobacterium tuberculosis,
pubmed-meshheading:8628661-Nucleic Acid Amplification Techniques
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pubmed:year |
1996
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pubmed:articleTitle |
DNA detection by strand displacement amplification and fluorescence polarization with signal enhancement using a DNA binding protein.
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pubmed:affiliation |
Becton Dickinson Research Center, Research Triangle Park, NC 27709-2016, USA.
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pubmed:publicationType |
Journal Article
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