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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1996-6-27
|
| pubmed:abstractText |
A stable chicken hybridoma secreting a monoclonal antibody (mAb) that detects the apical complex of Eimeria acervulina sporozoites has been developed by fusing a thymidine kinase (TK)-deficient chicken myeloma with spleen cells from chickens immunized with sporozoite antigen. The mAb, designated as 6D-12-G10, recognized the apical complex of a sporozoite of 20-21 kDa molecular mass on western blots. Immunoelectron microscopic examination revealed that mAb 6D-12-G10 stained the conoid antigen. Furthermore, mAb 6D-12-G10 inhibited the invasion of sporozoites into CD8+ T cells in vitro. These results suggest that the conoid may play an important role in the recognition and invasion of host cells by Eimeria sporozoites.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0022-3395
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
82
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
82-7
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8627507-Animals,
pubmed-meshheading:8627507-Antibodies, Monoclonal,
pubmed-meshheading:8627507-Antibody Specificity,
pubmed-meshheading:8627507-Antigens, Protozoan,
pubmed-meshheading:8627507-Blotting, Western,
pubmed-meshheading:8627507-CD8-Positive T-Lymphocytes,
pubmed-meshheading:8627507-Cells, Cultured,
pubmed-meshheading:8627507-Chickens,
pubmed-meshheading:8627507-Dose-Response Relationship, Immunologic,
pubmed-meshheading:8627507-Eimeria,
pubmed-meshheading:8627507-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:8627507-Hybridomas,
pubmed-meshheading:8627507-Microscopy, Immunoelectron,
pubmed-meshheading:8627507-Molecular Weight,
pubmed-meshheading:8627507-Multiple Myeloma,
pubmed-meshheading:8627507-Spleen,
pubmed-meshheading:8627507-Tumor Cells, Cultured
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Characterization of a chicken monoclonal antibody that recognizes the apical complex of Eimeria acervulina sporozoites and partially inhibits sporozoite invasion of CD8+ T lymphocytes in vitro.
|
| pubmed:affiliation |
Parasite Immunobiology Laboratory, United States Department of Agriculture, ARS Beltsville, Maryland 20705, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
|