Linked Life Data

8626737

Source:http://linkedlifedata.com/resource/pubmed/id/8626737

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1996-6-21
pubmed:databankReference
pubmed:abstractText
Paneth cells, secretory epithelial cells of the small intestinal crypts, are proposed to contribute to local host defense. Both mouse and human Paneth cells express a collection of antimicrobial proteins, including members of a family of antimicrobial peptides named defensins. In this study, data from an anchored polymerase chain reaction (PCR) strategy suggest that only two defensin mRNA isoforms are expressed in the human small intestine, far fewer than the number expressed in the mouse. The two isoforms detected by this PCR approach were human defensin family members, HD-5 and HD-6. The gene encoding HD-6 was cloned and characterized. HD-6 has a genomic organization similar to HD-5, and the two genes have a striking pattern of sequence similarity localized chiefly in their proximal 5'-flanking regions. Analysis of human fetal RNA by reverse transcriptase-PCR detected enteric defensin HD-5 mRNA at 13.5 weeks of gestation in the small intestine and the colon, but by 17 weeks HD-5 was restricted to the small intestine. HD-6 mRNA was detectable at 13.5-17 weeks of gestation in the small intestine but not in the colon. This pattern of expression coincides with the previously described appearance of Paneth cells as determined by ultrastructural approaches. Northern analysis of total RNA from small intestine revealed quantifiable enteric defensin mRNA in five samples from 19 24 weeks of gestation at levels approximately 40-250-fold less than those observed in the adult, with HD-5 mRNA levels greater than those of HD-6 in all samples. In situ hybridization analysis localized expression of enteric defensin mRNA to Paneth cells at 24 weeks of gestation, as is seen in the newborn term infant and the adult. Consistent with earlier morphological studies, the ratio of Paneth cell number per crypt was reduced in samples at 24 weeks of gestation compared with the adult, and this lower cell number partially accounts for the lower defensin mRNA levels as determined by Northern analysis. Low levels of enteric defensin expression in the fetus may be characteristic of an immaturity of local defense, which is thought to predispose infants born prematurely to infection from intestinal microorganisms.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
271
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4038-45
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8626737-Amino Acid Sequence, pubmed-meshheading:8626737-Animals, pubmed-meshheading:8626737-Anti-Infective Agents, pubmed-meshheading:8626737-Base Sequence, pubmed-meshheading:8626737-Blood Proteins, pubmed-meshheading:8626737-Cloning, Molecular, pubmed-meshheading:8626737-Colon, pubmed-meshheading:8626737-DNA Primers, pubmed-meshheading:8626737-Defensins, pubmed-meshheading:8626737-Embryonic and Fetal Development, pubmed-meshheading:8626737-Gene Expression Regulation, pubmed-meshheading:8626737-Humans, pubmed-meshheading:8626737-Intestinal Mucosa, pubmed-meshheading:8626737-Intestine, Small, pubmed-meshheading:8626737-Mice, pubmed-meshheading:8626737-Molecular Sequence Data, pubmed-meshheading:8626737-Polymerase Chain Reaction, pubmed-meshheading:8626737-Recombinant Proteins, pubmed-meshheading:8626737-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:8626737-Sequence Homology, Nucleic Acid
pubmed:year
1996
pubmed:articleTitle
Human enteric defensins. Gene structure and developmental expression.
pubmed:affiliation
Division of Human Genetics, The Children's Hospital of Philadelphia, Pennsylvania, 19104, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't