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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
19
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pubmed:dateCreated |
1996-6-27
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pubmed:abstractText |
Atherogenesis involves cellular immune responses and altered vascular smooth muscle cell (SMC) function. Cytokines such as interleukin (IL)-1 alpha and interferon-gamma (IFN-gamma) may contribute to this process by activating SMC. To determine whether the anti-atherogenic mediator, nitric oxide (.NO), can modulate cytokine-induced SMC activation, we investigated the effects of various .NO-generating compounds on the expression of intercellular and vascular cell adhesion molecules (ICAM-1 and VCAM-1). Induction of ICAM-1 expression by IL-1 alpha and VCAM-1 expression by IFN-gamma was attenuated by .NO donors but not by cGMP analogues. Nuclear run-on assays and transfection studies using various VCAM-1 promoter constructs linked to the chloramphenicol acetyl-transferase reporter gene showed that .NO repressed IFN-gamma-induced VCAM-1 gene transcription, in part, through inhibition of nuclear factor-kappa B (NF-kappa B). Electrophoretic mobility shift assay revealed that SMC possess basal constitutive NF-kappa B activity, which was augmented by treatment with IL-1 alpha. In contrast, IFN-gamma induced and activated interferon regulatory factor (IRF)-1 but had little effect on basal constitutive NF-kappa B activity. .NO donors had no inhibitory effect on IRF-1 activation but did inhibit basal and IL-1 alpha-stimulated NF-kappa B activation. These findings suggest that the induction of ICAM-1 and VCAM-1 expression requires NF-kappa B activation and that .NO attenuates IFN-gamma-induced VCAM-1 expression primarily by inhibiting basal constitutive NF-kappa B activity in SMC.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3-morpholino-sydnonimine,
http://linkedlifedata.com/resource/pubmed/chemical/8-Bromo Cyclic Adenosine...,
http://linkedlifedata.com/resource/pubmed/chemical/Chloramphenicol O-Acetyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Adhesion Molecule-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Molsidomine,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitroprusside,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Cell Adhesion Molecule-1
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
271
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11317-24
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8626684-8-Bromo Cyclic Adenosine Monophosphate,
pubmed-meshheading:8626684-Aorta,
pubmed-meshheading:8626684-Base Sequence,
pubmed-meshheading:8626684-Binding Sites,
pubmed-meshheading:8626684-Blotting, Northern,
pubmed-meshheading:8626684-Cell Nucleus,
pubmed-meshheading:8626684-Chloramphenicol O-Acetyltransferase,
pubmed-meshheading:8626684-Humans,
pubmed-meshheading:8626684-Intercellular Adhesion Molecule-1,
pubmed-meshheading:8626684-Interferon-gamma,
pubmed-meshheading:8626684-Interleukin-1,
pubmed-meshheading:8626684-Kinetics,
pubmed-meshheading:8626684-Molecular Sequence Data,
pubmed-meshheading:8626684-Molsidomine,
pubmed-meshheading:8626684-Muscle, Smooth, Vascular,
pubmed-meshheading:8626684-NF-kappa B,
pubmed-meshheading:8626684-Nitric Oxide,
pubmed-meshheading:8626684-Nitroprusside,
pubmed-meshheading:8626684-Oligodeoxyribonucleotides,
pubmed-meshheading:8626684-Promoter Regions, Genetic,
pubmed-meshheading:8626684-RNA, Messenger,
pubmed-meshheading:8626684-Recombinant Proteins,
pubmed-meshheading:8626684-Saphenous Vein,
pubmed-meshheading:8626684-Transcription, Genetic,
pubmed-meshheading:8626684-Transcription Factors,
pubmed-meshheading:8626684-Transfection,
pubmed-meshheading:8626684-Tunica Media,
pubmed-meshheading:8626684-Vascular Cell Adhesion Molecule-1
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pubmed:year |
1996
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pubmed:articleTitle |
Nitric oxide attenuates vascular smooth muscle cell activation by interferon-gamma. The role of constitutive NF-kappa B activity.
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pubmed:affiliation |
Cardiovascular Division, Brigham & Women's Hospital, Boston, Massachusetts, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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