Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
19
|
| pubmed:dateCreated |
1996-6-27
|
| pubmed:abstractText |
Syntaxin 1A is a nervous system-specific protein thought to function during the late steps of the regulated secretory pathway by mediating the docking of secretory vesicles with the plasma membrane. We have examined the effects of transiently overexpressing syntaxin 1A on protein secretion in constitutively secreting cell lines that do not normally express the protein. Syntaxin 1A showed the constitutive release of marker proteins human growth hormone (hGH) and vesicular stomatitis virus glycoprotein from COS-1 cells, increasing the intracellular half-life of human growth hormone from 90 min to 18 h. A similar effect was observed in HEK 293 cells. Immunofluorescence microscopy revealed that these secretory proteins were concentrated in the periphery of the cell. The effect was specific for the full-length neuronal protein. Neither a syntaxin 1A variant which lacks a membrane attachment domain nor syntaxin 2 caused the cells to retain human growth hormone. The effect of syntaxin 1A was partially reversed by incubating the cells with botulinum type C1 neurotoxin, which specifically cleaves syntaxin 1A. Release of human growth hormone from syntaxin 1A-expressing cells was maintained during a blockade of protein synthesis, suggesting that the hormone was being released from a pool of stored vesicles which accumulated before the addition of cycloheximide. The existence of a post-Golgi storage compartment in syntaxin 1A-expressing cells was confirmed using brefeldin A to collapse the Golgi stacks in both HEK 293 and COS-1 cells. Brefeldin A rapidly blocked growth hormone release in control cultures while having no effect on release in cells expressing syntaxin 1A. Reducing the temperature to 19 degrees C, which inhibits transport from the trans-Golgi network, also inhibited hGH secretion from cells without syntaxin 1A but had little effect on hGH secretion from cells with syntaxin 1A. The present experiments indicate that syntaxin 1A enables the storage of vesicles which would otherwise be immediately released.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Botulinum Toxins,
http://linkedlifedata.com/resource/pubmed/chemical/Brefeldin A,
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclopentanes,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Metallothionein,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neurotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Synthesis Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/STX1A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Stx1a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Syntaxin 1
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
10
|
| pubmed:volume |
271
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
11214-21
|
| pubmed:dateRevised |
2010-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:8626670-Animals,
pubmed-meshheading:8626670-Antigens, Surface,
pubmed-meshheading:8626670-Botulinum Toxins,
pubmed-meshheading:8626670-Brefeldin A,
pubmed-meshheading:8626670-Cell Line,
pubmed-meshheading:8626670-Cell Membrane,
pubmed-meshheading:8626670-Cercopithecus aethiops,
pubmed-meshheading:8626670-Cycloheximide,
pubmed-meshheading:8626670-Cyclopentanes,
pubmed-meshheading:8626670-Cytoplasmic Granules,
pubmed-meshheading:8626670-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:8626670-Gene Expression,
pubmed-meshheading:8626670-Growth Hormone,
pubmed-meshheading:8626670-Humans,
pubmed-meshheading:8626670-Kidney,
pubmed-meshheading:8626670-Kinetics,
pubmed-meshheading:8626670-Metallothionein,
pubmed-meshheading:8626670-Mice,
pubmed-meshheading:8626670-Nerve Tissue Proteins,
pubmed-meshheading:8626670-Neurotoxins,
pubmed-meshheading:8626670-Promoter Regions, Genetic,
pubmed-meshheading:8626670-Protein Synthesis Inhibitors,
pubmed-meshheading:8626670-Recombinant Proteins,
pubmed-meshheading:8626670-Syntaxin 1,
pubmed-meshheading:8626670-Transfection,
pubmed-meshheading:8626670-Vesicular stomatitis Indiana virus
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Evidence that syntaxin 1A is involved in storage in the secretory pathway.
|
| pubmed:affiliation |
Department of Pharmacology, University of Michigan Medical School, Ann Arbor 48109, USA. mbittner@umich.edu
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|