Linked Life Data

8626306

Source:http://linkedlifedata.com/resource/pubmed/id/8626306

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1996-6-27
pubmed:abstractText
For the first time, unidirectional rate constants of ethanol diffusion through the lipid membrane of a microorganism, the bacterium Zymomonas mobilis, were determined, thus replacing indirect inferences with direct kinetic data. The rate constants k1 (in to out) were 6.8 +/- 0.4s(-1) at 29 degrees C and 2.7 +/- 0.3s(-1) at 20 degrees C. They were determined by using 1H selective nuclear magnetic resonance spin magnetization transfer. The measurements were done on l-ml cell suspensions. No addition of radiotracers, withdrawing of aliquots, physical separation methods, or chemical manipulations were required. Until now, the rate constants of ethanol transport in microorganisms have been unknown because ethanol diffuses through the cytoplasmic membrane too quickly for radiolabel approaches. Net velocities of ethanol exchange were calculated from unidirectional rate constants and cytoplasmic volume, which was also determined with the same nuclear magnetic resonance experiments. The results (i) confirmed that ethanol would not be rate limiting during the conversion of glucose by Z. mobilis and (ii) indicated that ethanol can serve as an in vivo marker of cytoplasmic volume changes. This was verified by monitoring for the first time the changes of both cytoplasmic volume and extracytoplasmic and cytoplasmic concentrations of alpha and beta anomers of D-glucose in cell suspensions of a microorganism. These findings may open up new possibilities for kinetic studies of ethanol and sugar transport in Z. mobilis and other organisms.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8626306-1332937, http://linkedlifedata.com/resource/pubmed/commentcorrection/8626306-16346694, http://linkedlifedata.com/resource/pubmed/commentcorrection/8626306-16585, http://linkedlifedata.com/resource/pubmed/commentcorrection/8626306-2201390, http://linkedlifedata.com/resource/pubmed/commentcorrection/8626306-2248675, http://linkedlifedata.com/resource/pubmed/commentcorrection/8626306-2374002, http://linkedlifedata.com/resource/pubmed/commentcorrection/8626306-3078745, http://linkedlifedata.com/resource/pubmed/commentcorrection/8626306-3242610, http://linkedlifedata.com/resource/pubmed/commentcorrection/8626306-334768, http://linkedlifedata.com/resource/pubmed/commentcorrection/8626306-3513699, http://linkedlifedata.com/resource/pubmed/commentcorrection/8626306-5650082, http://linkedlifedata.com/resource/pubmed/commentcorrection/8626306-6090136, http://linkedlifedata.com/resource/pubmed/commentcorrection/8626306-6389514, http://linkedlifedata.com/resource/pubmed/commentcorrection/8626306-7768841, http://linkedlifedata.com/resource/pubmed/commentcorrection/8626306-8203901, http://linkedlifedata.com/resource/pubmed/commentcorrection/8626306-8489007
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
178
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1756-61
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Ethanol transport in Zymomonas mobilis measured by using in vivo nuclear magnetic resonance spin transfer.
pubmed:affiliation
Institut für Biotechnologie-1, Forschungszentrum Jülich, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't