Linked Life Data

8626020

Source:http://linkedlifedata.com/resource/pubmed/id/8626020

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1996-6-25
pubmed:databankReference
pubmed:abstractText
In this paper we present a structural and functional characterization of a new sea urchin embryo transcription factor, SpRunt-1. This factor was isolated by means of its specific interaction with a cis-regulatory target site of the CyIIIa gene. Here we show that this target site, the P7I site, is required for normal embryonic activation of CyIIIa x CAT reporter gene constructs. An oligonucleotide affinity column bearing the P7I target site purifies a 21-kDa polypeptide from blastula-stage nuclear extracts, and the amino acid sequence obtained from this polypeptide was used to generate a nucleic acid probe with which the corresponding cDNA was cloned. The cDNA encodes an approximately 60-kDa protein, SpRunt-1, which includes a "runt domain" that is closely homologous to those of Drosophila and mammalian runt domain transcription factors. RNA and genomic blots show that SpRunt-1 is represented by a single embryonic transcript, encoded by one of possibly two runt-domain-containing genes. By RNA probe protection we found that transcripts of SpRunt-1 increase in concentration dramatically after the blastula stage of development, suggesting that the up-regulation of CyIIIa that occurs after blastula stage is a function of zygotically transcribed SpRunt-1. These results are discussed with reference to known features of the runt domain family of transcription factors.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0012-1606
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
174
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
43-54
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8626020-Actins, pubmed-meshheading:8626020-Amino Acid Sequence, pubmed-meshheading:8626020-Animals, pubmed-meshheading:8626020-Base Sequence, pubmed-meshheading:8626020-DNA-Binding Proteins, pubmed-meshheading:8626020-Gene Expression Regulation, Developmental, pubmed-meshheading:8626020-Humans, pubmed-meshheading:8626020-Mice, pubmed-meshheading:8626020-Molecular Sequence Data, pubmed-meshheading:8626020-Oligonucleotide Probes, pubmed-meshheading:8626020-RNA, Messenger, pubmed-meshheading:8626020-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:8626020-Sea Urchins, pubmed-meshheading:8626020-Sequence Alignment, pubmed-meshheading:8626020-Sequence Homology, Amino Acid, pubmed-meshheading:8626020-Transcription, Genetic, pubmed-meshheading:8626020-Transcription Factors
pubmed:year
1996
pubmed:articleTitle
SpRunt-1, a new member of the runt domain family of transcription factors, is a positive regulator of the aboral ectoderm-specific CyIIIA gene in sea urchin embryos.
pubmed:affiliation
Division of Biology 156-29, California Institute of Technology, Pasadena, California 91125, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't